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- Breast Skin Temperature Evaluation in Lactating and Non-lactating Women by Thermography: An Exploratory StudyPublication . Gouveia, Ana; Pires, Luís Carlos Carvalho; Garcia, Nuno; Barbosa, Ana; Jesus, Ana; Pombo, Nuno; Soares, Marta; Oliveira, J. Martinez deDuring pregnancy and lactation, woman breasts feel changes like blood flow increasement, associated with a higher breast temperature. We performed an exploratory study of the breast skin temperature of lactating and non-lactating women based on thermography, with a qualitative analysis of the temperature patterns and a quantitative evaluation of the differences. Frontal breast thermograms of four non-lactating young women and four women with well-established lactation were acquired and analyzed. Qualitative analysis of the images obtained show some evidence of the existence of a characteristic skin temperature pattern for lactating women. Quantitative differences between thermograms were also noticed, especially when considering dispersion metrics: lactating women present higher breast skin temperature gradients and amplitudes. Results obtained, especially based on central tendency metrics, should be interpreted with caution because some of the acquisition conditions for non-lactating women may lead to some bias on the results. Further investigation will be performed to quantify breast skin temperature gradient and be able to classify images based in the breast skin temperature pattern.
- Wearable Sensors for Foetal Movement Monitoring in Low Risk PregnanciesPublication . Borges, Luís M.; Araújo, Pedro; Lebres, António S.; Rente, Andreia; Salvado, Rita; Velez, Fernando J.; Martinez-de-Oliveira, J.; Barroca, Norberto; Ferro, João MiguelIn low risk pregnancies, the continuous monitoring of the foetal health is based on traditional protocols for counting the foetal movements felt by the mother. Although the maternal perception is a relevant characteristic for the evaluation of the foetal health, this kind of monitoring is hard to accomplish and being subjective can induce into errors due to mother’s anxiety and lack of concentration. Furthermore, the majority of foetal fatalities occur during the last weeks of low risk pregnancies. Therefore, it is important to obtain a universal electronic obstetric tracing, allowing for the identification of sudden changes in the foetus health, by continuously monitoring the foetus movements. The Smart-Clothing project aim has been the development of easy-to-wear belts with a telemedicine system for this purpose. One of the tried solutions is the Flex sensor belt system, which guarantees real-time and continuous foetal monitoring while creating effective interfaces for querying sensor data and store all the medical record (which can later be accessed by health professionals). Another developed belt has piezoelectric sensors incorporated onto it. The piezoelectric sensor belt has shown a high capacity to detect foetal movements, isolating them from external interferences.
- Bacteriocin production of the probiotic Lactobacillus acidophilus KS400Publication . Gaspar, C; Donders, Gilbert; Oliveira, Rita Palmeira de; Queiroz, João; Tomaz, C. T.; Oliveira, J. Martinez de; Oliveira, Ana Palmeira deIn the last years, the use of probiotics, including Lactobacillus species, has received much attention to prevent and treat vaginal disorders. These species have been described as having the ability to colonize the epithelial surface and produce antimicrobial metabolites that are able to control the remaining vaginal microflora. This study aimed to identify and characterize, for the first time, a bacteriocin natively produced by Lactobacillus acidophilus KS400 (probiotic strain from Gynoflor®-Medinova AG, Switzerland) and its antimicrobial activity against relevant urogenital pathogens. After organic acids and hydrogen peroxide neutralization in the fermented Lactobacillus acidophilus KS400 culture medium, bacteriocin activity was tested against the indicator microorganism Lactobacillus delbrueckii ATCC9649. The fermentation of Lactobacillus acidophilus KS400 for bacteriocin production was carried out in batch mode, and its antimicrobial activity, optical density and pH were monitored. After production and extraction, the bacteriocin molecular weight was estimated by electrophoresis and tested against vaginal pathogenic microorganisms. As described for other bacteriocins, batch fermentation profiles indicated that bacteriocin production occurs during the exponential growth phase of the lactobacilli, and declines during their stationary growth phase. The molecular weight of the bacteriocin is approximately 7.5 kDa. The bacteriocin containing protein extract was shown to inhibit the growth of Gardnerella vaginalis, Streptococcus agalactiae, Pseudomonas aeruginosa and the indicator strain Lactobacillus delbrueckii ATCC9649. We conclude that L. acidophilus KS400 produces bacteriocin with antimicrobial activity against relevant urogenital pathogens.
- The role of marketing in the promotion of breastfeedingPublication . Paço, Arminda; Rodrigues, Ricardo; Duarte, Paulo; Pinheiro, Paulo; Oliveira, José António Martinez Souto de; Soares, MartaIn recent years, researchers and health-care professionals have shown a growing interest in the health benefits associated with breastfeeding and the dangers associated with bottle feeding. At the same time, there has been a growing number of medical and academic studies showing that breastfeeding is beneficial and necessary. However, despite its being a socially important theme, little research has been undertaken in the marketing of breastfeeding. The purpose of this article is to review the use of social marketing as a tool for enhancing health promotion programmes, specifically those seeking to encourage the adoption of breastfeeding and to increase its prevalence. The implementation of the first phase of such a programme is described in the article. Based on a sample of women, this study illustrates mothers' knowledge of breastfeeding, showing that social marketing programmes are needed to improve their level of access to information and increase the prevalence of breastfeeding.
- UV-B Filter Octylmethoxycinnamate Induces Vasorelaxation by Ca2+ Channel Inhibition and Guanylyl Cyclase Activation in Human Umbilical ArteriesPublication . Lorigo, Margarida; Antunes, Carla; Lemos, Manuel C.; Oliveira, J. Martinez de; Granadeiro, Luiza Breitenfeld; Cairrão, ElisaUltraviolet (UV) filters are chemicals widely used in personal care products (PCPs). Due to their effect as endocrine disruptor compounds (EDCs), the toxicity of UV filters is a current concern for human health. EDC exposure may be correlated to cardiovascular diseases (CVD), but to our knowledge, no studies assessed the UV filters effects as human EDCs at the vascular level. Octylmethoxycinnamate (OMC) is the world's most widely used UV-B filter, present in more than 90% of PCPs. Due to its demonstrated multiple hormonal activities in animal models, this substance is also suspected to be a human EDC. The purpose of this study was to assess the rapid/short-term effects of OMC on arterial tonus and analyse its mode of action (MOA). Using human umbilical arteries, the endocrine effects of OMC were evaluated in in vitro (cellular and organ) experiments by planar cell surface area (PCSA) and organ bath, respectively. Our data show that OMC induces a rapid/short-term smooth muscle relaxation acting through an endothelium-independent MOA, which seems to be shared with oestrogens, involving an activation of soluble guanylyl cyclase (sGC) that increases the cyclic guanosine monophosphate (cGMP) intracellular levels and an inhibition of L-type voltage-operated Ca2+ channels (L-Type VOCC).
- Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profilePublication . Oliveira, Ana Palmeira de; Ribeiro, MP.; Oliveira, Rita Manuela Palmeira de; Gaspar, Carlos; Oliveira, Sofia Costa de; Correia, I.J.; Vaz, Cidália Pina; Oliveira, José António Martinez Souto de; Queiroz, João; Rodrigues, AcácioCandida spp. are common causative agents of mucocutaneous infections. New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols. Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties. The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts. CH antifungal activity was assessed according to CLSI reference M27-A3 protocol; its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay. CH demonstrated a full inhibition of C. tropicalis, C. krusei, C. guilliermondii and C. parapsilosis growth while impairing C. albicans and C. glabrata viability. Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane. However, CH showed no cytotoxic effect upon human fibroblasts cells. Resistant strains will require new therapeutic approaches. Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis.
- Sexual expression involving people with dementia living in long‐term care facilities: staff's reactionsPublication . Fonseca, Maria Isabel; Almeida, Daniela; Martins, Ana Paula; Cerqueira, Margarida; Villar, Feliciano; Oliveira, José Martinez de; Afonso, Rosa MarinaThis study aims to explore and compare the reaction of long-term care staff towards situations of sexual intercourse in which one or both partners have dementia. Participants were 538 staff members at 28 Portuguese long-term care facilities. Data were collected using a printed questionnaire with seven vignettes with situations related to the sexuality of LTCF residents. In this study, three of these vignettes were analysed with situations in which residents were having relationships in their bedrooms: (1) one male resident and one female resident; (2) two residents with dementia; (3) two residents, one of them with dementia. For each of the vignettes, participants are asked what they think their ‘colleagues’ would do in each vignette, i.e. ‘What do you think most of your colleagues would do?’ The results revealed that when sexual relationships involved both partners living with dementia, the most selected reaction was ‘Comment on what happened with supervision or direction’, which may suggest that the situation was perceived as difficult or problematic. The most restrictive reactions were most often chosen when only one partner had dementia. This study suggests the need to improve and provide training, so that staff have knowledge and strategies that allow people living with dementia to preserve their sexual rights in long-term care facilities.
- Chemical signature and antimicrobial activity of Central Portuguese Natural Mineral Waters against selected skin pathogensPublication . Oliveira, AS; Vaz, CV; Silva, Ana; Ferreira, Sandra S.; Correia, Sara; Ferreira, Raquel; Granadeiro, Luiza Breitenfeld ; Oliveira, J. Martinez de; Oliveira, Rita Palmeira de; Pereira, C; Cruz, MT; Oliveira, Ana Palmeira deThe common therapeutic indications of Portuguese Natural Mineral Waters (NMWs) are primarily for respiratory, rheumatic and muscu- loskeletal systems. However, these NMWs have been increasingly sought for dermatologic purposes. Opposing to what is observed in the major European Thermal Centres, there are few scientific evidences supporting the use of Portuguese NMWs for clinical applications. The aim of this study was to characterize the antimicrobial profile of individual NMWs from the central region of Portugal and correlate the results with their physicochemical characterization. An extensive multivariate analysis (principal component analysis) was also performed to further investigate this possible correlation. Six collection strains representing skin microbiota, namely Staphylococcus aureus, Escher- ichia coli, Corynebacterium amycolatum, Candida albicans, Staphylococcus epidermidis and Cutibac- terium acnes, were analysed, and their antimicrobial profile was determined using Clinical and Laboratory Standards Institute M07-A10, M45-A2, M11-A6 and M27-A3 microdilution methods. Different NMWs presented different antimicrobial profiles against the strains used; the physicochemical composition of NMWs seemed to be correlated with the different susceptibility profiles. Cutibacterium acnes showed a particularly high susceptibility to all NMWs belong- ing sulphurous/bicarbonated/sodic ionic profile, exhibiting microbial reductions up to 65%. However, due to the complex physicochemical composition of each water an overall conclusion regarding the effect of a specific ion on the growth of different microor- ganisms is yet to be known.