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Authors
Abstract(s)
O presente trabalho encontra-se dividido em 3 capítulos nos quais são demonstradas não só as
atividades desenvolvidas e conhecimentos adquiridos ao longo dos dois estágios curriculares,
nas áreas de farmácia hospitalar e farmácia comunitária, mas também o trabalho laboratorial
desenvolvido nas áreas da fitoquímica e microbiologia.
O primeiro capítulo pretende retratar o período inicial de estágio, que decorreu entre os meses
de janeiro e março de 2019 nos Serviços Farmacêuticos da Unidade Local de Saúde de Castelo
Branco. Durante o período de estágio foram abordadas as várias áreas existentes, que de forma
genérica se podem dividir em gestão, distribuição, farmacotecnia e ambulatório, sendo que
dentro de cada uma destas foram focados não só os conhecimentos teóricos e técnicocientíficos,
mas também as boas práticas e o carácter humano que deve pautar a atuação do
farmacêutico enquanto profissional de saúde, independentemente do local de atuação.
No segundo capítulo intenta-se, de forma semelhante, transpor o período de estágio decorrido
entre março e maio de 2019, na Farmácia Ferrer, em Castelo Branco. Ao longo das 12 semanas
foram abordadas diversas áreas, desde a receção das encomendas até ao atendimento ao
público, passando também pelos serviços prestados aos utentes. A importância e o papel do
farmacêutico enquanto profissional de saúde, a sua atuação e contacto com o utente
constituíram pilares de extrema importância durante toda a aprendizagem.
Por último, o terceiro capítulo pretende demonstrar todo o trabalho laboratorial desenvolvido
no Centro de Biotecnologia de Plantas da Beira Interior, bem como na Escola Superior Agrária
de Castelo Branco. Sendo a resistência aos antibióticos um dos principais problemas na saúde
no século XXI, torna-se imperativo descobrir novos compostos que demonstrem capacidade de
inibir o crescimento dos microrganismos. As atividades desenvolvidas tiveram como principal
objetivo avaliar o potencial antibacteriano da maçã Bravo-de-Esmolfe (Malus domestica cv.
Bravo-de-Esmolfe). Inicialmente procedeu-se à preparação da amostra, de forma a avaliar
possíveis diferenças entre os compostos existentes na casca e na polpa da fruta. Após a
obtenção de extratos com recurso a dois solventes distintos, bem como diversas técnicas
extrativas, procedeu-se à avaliação da atividade antimicrobiana contra cinco microrganismos.
Após a realização de ensaios em meio sólido, foram determinadas as concentrações mínimas
inibitórias dos vários extratos brutos em meio líquido Müeller-Hinton, sendo que o resultado
mais baixo obtido foi de 31,25 mg/mL para a estirpe de Bacillus cereus. Com a determinação
das concentrações mínimas bactericidas foi possível concluir que os extratos obtidos apenas
possuem ação inibitória, não sendo capazes de matar os microrganismos.
The current work is divided in 3 chapters in which are demonstrated not only the developed activities and aquired knowledges throughout the two internships, in community and hospital pharmacy, but also the laboratorial work builded up in the phytochemistry and microbiology fields. The first chapter intends to describe the first intership period, which begun in january until march 2019, at the Pharmaceutical Services of the Unidade Local de Saúde de Castelo Branco. During the internship period, the various existing areas were approached, which can be divided into management, distribution, pharmacotechnics and ambulatory, and within each of these were focused not only theoretical and technical-scientific knowledges, but also good practices and the human character that should guide the pharmacist's performance as a healthcare professional, regardless of the place of performance. The second chapter seeks, similarly, to transpose the internship period between march to may 2019 at the Farmácia Ferrer, in Castelo Branco. Over the 12 weeks various areas were addressed, from receiving orders to serving the public, as well as services to users. The importance and role of the pharmacist as a health professional, his performance and contact with the user were extremely important pillars throughout the learning process. Finally, the third chapter aims to demonstrate all the laboratory work developed at the Beira Interior Plant Biotechnology Center, as well as at the Castelo Branco School of Agriculture. As antibiotic resistance is one of the main health problems in the 21st century, it is imperative to discover new molecules that demonstrate the ability to inhibit the microorganisms’ growth. The activities that were developed had as main goal the evaluation of the antibacterial potential of Bravo-de-Esmolfe apple (Malus domestica cv. Bravo-de-Esmolfe). Initially, the sample was prepared in order to evaluate possible differences between the peel and fruit pulp existing compounds. After obtaining extracts using two distinct solvents, as well as two different extractive techniques, the antimicrobial activity against five microorganisms was evaluated. After testing in solid medium, the minimum inhibitory concentrations of the various crude extracts were determined in Müeller-Hinton broth, and the lowest result obtained was 31.25 mg / mL for the Bacillus cereus strain. With the determination of the minimum bactericidal concentrations it was possible to conclude that the obtained extracts only have inhibitory action, not being able to kill the microorganisms.
The current work is divided in 3 chapters in which are demonstrated not only the developed activities and aquired knowledges throughout the two internships, in community and hospital pharmacy, but also the laboratorial work builded up in the phytochemistry and microbiology fields. The first chapter intends to describe the first intership period, which begun in january until march 2019, at the Pharmaceutical Services of the Unidade Local de Saúde de Castelo Branco. During the internship period, the various existing areas were approached, which can be divided into management, distribution, pharmacotechnics and ambulatory, and within each of these were focused not only theoretical and technical-scientific knowledges, but also good practices and the human character that should guide the pharmacist's performance as a healthcare professional, regardless of the place of performance. The second chapter seeks, similarly, to transpose the internship period between march to may 2019 at the Farmácia Ferrer, in Castelo Branco. Over the 12 weeks various areas were addressed, from receiving orders to serving the public, as well as services to users. The importance and role of the pharmacist as a health professional, his performance and contact with the user were extremely important pillars throughout the learning process. Finally, the third chapter aims to demonstrate all the laboratory work developed at the Beira Interior Plant Biotechnology Center, as well as at the Castelo Branco School of Agriculture. As antibiotic resistance is one of the main health problems in the 21st century, it is imperative to discover new molecules that demonstrate the ability to inhibit the microorganisms’ growth. The activities that were developed had as main goal the evaluation of the antibacterial potential of Bravo-de-Esmolfe apple (Malus domestica cv. Bravo-de-Esmolfe). Initially, the sample was prepared in order to evaluate possible differences between the peel and fruit pulp existing compounds. After obtaining extracts using two distinct solvents, as well as two different extractive techniques, the antimicrobial activity against five microorganisms was evaluated. After testing in solid medium, the minimum inhibitory concentrations of the various crude extracts were determined in Müeller-Hinton broth, and the lowest result obtained was 31.25 mg / mL for the Bacillus cereus strain. With the determination of the minimum bactericidal concentrations it was possible to conclude that the obtained extracts only have inhibitory action, not being able to kill the microorganisms.
Description
Keywords
Atividade Antimicrobiana Bravo-de-Esmolfe Extratos Farmácia Comunitária Farmácia Hospitalar Malus Domestica