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Abstract(s)
Introdução: O bcl-2 é um gene que codifica um conjunto de proteínas com funções pró-apoptóticas e anti-apoptóticas. A proteína BCL-2 é uma dessas proteínas e, quando em níveis mais elevados pode promover imunidade celular à apoptose.
Associando estas capacidades neoplásicas da BCL-2 ao adenocarcinoma do cólon, um dos mais frequentes carcinomas no país e uma séria causa de morbilidade em Portugal, foi realizado um estudo em 29 pacientes a quem foi realizada cirurgia curativa de excisão de adenocarcinoma do cólon no CHCB.
Objetivo: Verificar se a presença da oncoproteína BCL-2 em células neoplásicas é um fator preditivo do prognóstico, verificando também se é preditivo do estadiamento, localização e diferenciação celular no adenocarcinoma colorretal.
Método: Serão estudados 29 pacientes com Adenocarcinoma Colorretal, submetidos a excisão cirúrgica com intenção curativa entre 2003 e 2005. Estes doentes foram sujeitos a um follow-up pós-operatório, nos 10 anos seguintes.
As amostras neoplásicas foram estudadas relativamente à sua localização e classificação TNM. Além disso, amostras foram sujeitas a um protocolo definido pelo Hospital Pêro da Covilhã de estudo imunohistoquímico da marcação da proteína Bcl-2. Posteriormente, as amostras foram classificadas de acordo com a intensidade e percentagem de células marcadas. Ao longo do estudo as amostras foram também classificadas de acordo com o seu grau de diferenciação histológico.
Com estes dados pretende-se realizar um estudo estatístico que tenta avaliar se existe uma correlação entre a marcação histoquímica de bcl-2 e o prognóstico do cancro colorretal, utilizando p=0.05 como significativamente estatístico.
Introduction: bcl-2 is a gene that codifies a group of proteins with pro-apoptotic and anti-apoptotic functions. BCL-2 protein is one of those and, when produced in higher levels, may promote cell immunity to apoptosis. Translating this BCL-2 neoplasic functions to colon adenocarcinoma, one of the most frequent cancers in the country and a serious morbidity cause in Portugal, a study was realized about 29 pacients, to whom was realized a curative colon adenocarcinoma excision cirurgy. Objective: Verify if the the BCL-2 protein presence in neoplasic cells is a predictive factor of the prognosis, verifying also if it predicts cell staging, lesion location and cell differentiation in colorectal adenocarcinoma. Method: 29 pacients with colorectal adenocarcinoma and subject to cure intenteded cirurgic excision from the year 2003 to 2005 will be studied. These pacients were subjet to a 10 years pos-op follow-up. Neoplasic samples were studied about its colon location and TNM classification. Also, these samples underwent an immunohistochimical marking protocol defined by Hospital Pêro da Covilhã to mark the protein Bcl-2. Afterwards they were classified according the intensity and percentage of marked cells. Cells from the samples were also studied about its histological differentiation. With this data, there will be realized a statistic study trying to evaluate if there is a correlation between immunohistochimical marking of Bcl-2 and the prognosis of colorectal adenocarcinoma, using p=0.05 as statistical reference.
Introduction: bcl-2 is a gene that codifies a group of proteins with pro-apoptotic and anti-apoptotic functions. BCL-2 protein is one of those and, when produced in higher levels, may promote cell immunity to apoptosis. Translating this BCL-2 neoplasic functions to colon adenocarcinoma, one of the most frequent cancers in the country and a serious morbidity cause in Portugal, a study was realized about 29 pacients, to whom was realized a curative colon adenocarcinoma excision cirurgy. Objective: Verify if the the BCL-2 protein presence in neoplasic cells is a predictive factor of the prognosis, verifying also if it predicts cell staging, lesion location and cell differentiation in colorectal adenocarcinoma. Method: 29 pacients with colorectal adenocarcinoma and subject to cure intenteded cirurgic excision from the year 2003 to 2005 will be studied. These pacients were subjet to a 10 years pos-op follow-up. Neoplasic samples were studied about its colon location and TNM classification. Also, these samples underwent an immunohistochimical marking protocol defined by Hospital Pêro da Covilhã to mark the protein Bcl-2. Afterwards they were classified according the intensity and percentage of marked cells. Cells from the samples were also studied about its histological differentiation. With this data, there will be realized a statistic study trying to evaluate if there is a correlation between immunohistochimical marking of Bcl-2 and the prognosis of colorectal adenocarcinoma, using p=0.05 as statistical reference.
Description
Keywords
Adenocarcinoma Colorretal Bcl-2 Cancro Colorretal Imunohistoquímica Prognóstico