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Authors
Abstract(s)
A presente dissertação insere-se na unidade curricular “Estágio” do Mestrado Integrado
em Ciências Farmacêuticas, e encontra-se dividida em dois capítulos.
O primeiro capítulo refere-se à vertente de investigação e descreve o desenvolvimento
de um método para a análise de compostos fenólicos presentes em mirtilos, utilizando
a cromatografia líquida de alta eficiência acoplada a um detetor de diode array (HPLCDAD). Este trabalho foi desenvolvido devido à cooperação do Centro de Investigação em
Ciências da Saúde (CICS) da Universidade da Beira Interior e a Escola Superior Agrária
do Instituto Politécnico de Castelo Branco.
Foram estudadas diferentes condições cromatográficas de forma a ter um método
seletivo para os compostos em estudo: ácidos cafeíco, clorogénico, elágico, ferrúlico,
gálico, p-cumárico, siríngico e vanílico, canferol, cianidina, epicatequina, laricitrina,
malvidina, peonidina, quercetina, resveratrol, rutina, e siringetina. O método otimizado
foi aplicado a amostras reais de forma a avaliar a aplicabilidade do método.
O segundo capítulo diz respeito ao estágio em Farmácia Comunitária, que teve lugar na
Farmácia Reis, em Castelo Branco, no período compreendido entre 1 de fevereiro e 11
de junho de 2021. Este capítulo descreve não só as funções e responsabilidades de um
farmacêutico comunitário e o seu enquadramento legal, mas também a minha
experiência enquanto estagiária e os conhecimentos adquiridos ao longo de cerca de
quatro meses e meio.
The present dissertation is part of the curricular unit “Internship” of the Integrated Master in Pharmaceutical Sciences, and is divided into two chapters. The first chapter refers to the Investigation experience, and describes the development of a qualitative method for the analysis of phenolic compounds in blueberries, using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). This work was developed within the cooperation between the Centro de Investigação em Ciências da Saúde (CICS) of the Universidade da Beira Interior and the Escola Superior Agrária of the Instituto Politécnico de Castelo Branco. Different chromatographic conditions were studied in order to develop a selective method for the compounds under study: caffeic, chlorogenic, ellagic, ferrulic, gallic, p-coumaric, syringic and vanillic acids, kaempferol, cyanidin, epicatechin, laricitrin, malvidin, peonidin, quercetin, resveratrol, rutin and syringetin. The optimized method was applied to real samples in order to assess its applicability. The second chapter concerns the internship in community pharmacy which took place at Farmácia Reis, in Castelo Branco, between February 1st and June 11th, 2021. This chapter describes not only the roles and responsibilities of a community pharmacist and his legal framework, but also my experience as an intern and the knowledge acquired over about four and a half months.
The present dissertation is part of the curricular unit “Internship” of the Integrated Master in Pharmaceutical Sciences, and is divided into two chapters. The first chapter refers to the Investigation experience, and describes the development of a qualitative method for the analysis of phenolic compounds in blueberries, using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). This work was developed within the cooperation between the Centro de Investigação em Ciências da Saúde (CICS) of the Universidade da Beira Interior and the Escola Superior Agrária of the Instituto Politécnico de Castelo Branco. Different chromatographic conditions were studied in order to develop a selective method for the compounds under study: caffeic, chlorogenic, ellagic, ferrulic, gallic, p-coumaric, syringic and vanillic acids, kaempferol, cyanidin, epicatechin, laricitrin, malvidin, peonidin, quercetin, resveratrol, rutin and syringetin. The optimized method was applied to real samples in order to assess its applicability. The second chapter concerns the internship in community pharmacy which took place at Farmácia Reis, in Castelo Branco, between February 1st and June 11th, 2021. This chapter describes not only the roles and responsibilities of a community pharmacist and his legal framework, but also my experience as an intern and the knowledge acquired over about four and a half months.
Description
Keywords
Compostos Fenólicos Farmácia Comunitária Hplc-Dad Mirtilos