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Authors
Abstract(s)
O presente relatório de estágio para obtenção do grau de Mestre em Ciências
Farmacêuticas foi realizado no âmbito da Unidade Curricular “Estágio” do Mestrado
Integrado em Ciências Farmacêuticas da Universidade da Beira Interior (Covilhã). O
relatório de estágio apresentado é constituído por dois capítulos.
O primeiro é referente à componente de investigação laboratorial, desenvolvida no
Centro de Investigação em Ciências da Saúde da Universidade da Beira Interior. O
intuito do presente trabalho foi validar e aplicar um método analítico para a
determinação de canabinóides em extratos de chá, bebidas e óleos orais por
cromatografia líquida de elevada eficiência acoplada a um detetor de fotododiodos.
Foram analisadas amostras de extratos de chá, óleos orais e bebidas. A metodologia
desenvolvida foi validada segundo normas de validação apresentados pela Food and
Drug Administration e pela European Medicines Agency, sendo os parâmetros
estudados a seletividade, linearidade, limites de quantificação e deteção, precisão,
exatidão, recuperação e estabilidade. O método validado foi aplicado a amostras reais, e
posteriormente, foram comparados os valores de teor em canabinóides obtidos pelo
método com os valores postulados nos rótulos das amostras recolhidas de produtos
comercializados. Neste estudo, na maioria dos casos, os resultados obtidos da
composição e teor de canabinóides nas amostras, especialmente dos óleos,
apresentaram-se sobre-rotulados. Quatro das dezanove amostras analisadas não
apresentavam informações detalhadas sobre a composição e concentrações de
canabinóides nos rótulos. As amostras dos chás apenas diziam apresentar uma
composição de THC inferior a 0,02% (em conformidade com o legislado). E em todas
estas amostras, esta premissa foi confirmada. Por fim, no que concerne as amostras das
bebidas, os rótulos estavam em conformidade uma vez que tinham descrito na lista de
ingredientes que apenas continham aroma de canábis e, como esperado, nenhum
canabinóide foi detetado. Neste estudo concluímos então que, de um modo geral, a
concentração de CBD determinada nas amostras foi inferior à rotulada, o que pode
anular qualquer potencial resposta clínica.
O segundo capítulo diz respeito ao estágio curricular em Farmácia Comunitária,
realizado na farmácia Covilhã, o qual teve início a 8 de fevereiro de 2023 e término a 23
de junho de 2023, sob orientação da Dra. Carmo Santos. Neste capítulo, são abordadas
as atividades desenvolvidas, os conhecimentos adquiridos, as funções desempenhadas
em farmácia, a sua organização e os aspetos legais referentes a esta área. Esta oportunidade permitiu presenciar e participar no quotidiano de um farmacêutico
comunitário, testemunhando o seu impacto na comunidade.
The present internship report for the master’s degree in Pharmaceutical Sciences was carried out within the scope of the course "Internship" of the Integrated Master's in Pharmaceutical Sciences at the University of Beira Interior (Covilhã). The internship report presented consists of two chapters. The first chapter concerns the laboratory research component, conducted at the Health Sciences Research Center of the University of Beira Interior. The purpose of this work was to validate and apply an analytical method for the determination of cannabinoids in tea extracts, beverages, and oils by high-performance liquid chromatography coupled with a photodiode detector. Samples of tea extracts, consumable oils, and beverages were analyzed. The developed methodology was validated according to validation standards presented by the Food and Drug Administration and the European Medicines Agency. The parameters studied included selectivity, linearity, limits of quantification and detection, precision, accuracy, recovery, and stability. The validated method was applied to real samples, and subsequently, the cannabinoid content values obtained by the method were compared with the values stated on the labels of the collected commercially available products. In this study, in most cases, the results obtained for the composition and content of cannabinoids in the samples, especially the oils, were over-labeled. Four out of the nineteen samples analyzed did not provide detailed information about the composition and concentrations of cannabinoids on the label. The tea samples only claimed to have a THC composition of less than 0.02% (in accordance with the law). And in all these samples, this premise was confirmed. Finally, as far as the drink samples were concerned, the labels were compliant since they had described in the list of ingredients that they only contained cannabis flavoring and as expected, no cannabinoids were detected. In this study, we concluded that, in general, the concentration of CBD determined in the samples was lower than that labeled. The second chapter concerns the curricular internship in Community Pharmacy, carried out at the Covilhã pharmacy, which began on February 8, 2023 and ended on June 23, 2023, under the guidance of Dr. Carmo Santos. This chapter covers the activities carried out, the knowledge acquired, the functions performed in the pharmacy, its organization and the legal aspects relating to this area. This opportunity allowed me to witness and participate in the daily life of a community pharmacist, witnessing their impact on the community.
The present internship report for the master’s degree in Pharmaceutical Sciences was carried out within the scope of the course "Internship" of the Integrated Master's in Pharmaceutical Sciences at the University of Beira Interior (Covilhã). The internship report presented consists of two chapters. The first chapter concerns the laboratory research component, conducted at the Health Sciences Research Center of the University of Beira Interior. The purpose of this work was to validate and apply an analytical method for the determination of cannabinoids in tea extracts, beverages, and oils by high-performance liquid chromatography coupled with a photodiode detector. Samples of tea extracts, consumable oils, and beverages were analyzed. The developed methodology was validated according to validation standards presented by the Food and Drug Administration and the European Medicines Agency. The parameters studied included selectivity, linearity, limits of quantification and detection, precision, accuracy, recovery, and stability. The validated method was applied to real samples, and subsequently, the cannabinoid content values obtained by the method were compared with the values stated on the labels of the collected commercially available products. In this study, in most cases, the results obtained for the composition and content of cannabinoids in the samples, especially the oils, were over-labeled. Four out of the nineteen samples analyzed did not provide detailed information about the composition and concentrations of cannabinoids on the label. The tea samples only claimed to have a THC composition of less than 0.02% (in accordance with the law). And in all these samples, this premise was confirmed. Finally, as far as the drink samples were concerned, the labels were compliant since they had described in the list of ingredients that they only contained cannabis flavoring and as expected, no cannabinoids were detected. In this study, we concluded that, in general, the concentration of CBD determined in the samples was lower than that labeled. The second chapter concerns the curricular internship in Community Pharmacy, carried out at the Covilhã pharmacy, which began on February 8, 2023 and ended on June 23, 2023, under the guidance of Dr. Carmo Santos. This chapter covers the activities carried out, the knowledge acquired, the functions performed in the pharmacy, its organization and the legal aspects relating to this area. This opportunity allowed me to witness and participate in the daily life of a community pharmacist, witnessing their impact on the community.
Description
Keywords
Canabinóides Canábis Estágio Farmácia Comunitária Hplc-Dad Uso Recreativo