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Abstract(s)
O trabalho apresentado neste relatório provém do conhecimento adquirido e experienciado ao longo da passagem pelas três vertentes durante o estágio curricular do Mestrado Integrado em Ciências Farmacêuticas. O presente relatório encontra-se dividido em três capítulos, no qual cada capítulo diz respeito a cada uma das vertentes referidas anteriormente.
No primeiro capítulo, é abordada a experiência profissional adquirida durante o estágio de farmácia comunitária, realizado na Farmácia Taborda, situada na cidade do Fundão. Assim, procede-se à descrição de todos os competências e conhecimentos adquiridos durante o estágio, tendo em conta a informação descrita nas Boas Práticas em Farmácia Comunitária e a legislação regente da atividade.
No segundo capítulo, faz-se referência à experiência profissional adquirida durante o estágio da farmácia hospitalar desenvolvido no Hospital Sousa Martins da Unidade Local de Saúde da Guarda, encontrando-se descritas as atividades realizadas durante o estágio, relativamente ao papel desempenhado pelo farmacêutico hospitalar, conforme o descrito na legislação em vigor.
No terceiro capítulo encontra-se descrito o trabalho de investigação desenvolvido no Laboratório de “Síntese Orgânica” do Departamento de Química da Faculdade de Ciências e no Centro de Investigação em Ciências da Saúde (CICS) durante o último ano de frequência universitária. O alvo deste trabalho foi a síntese, caracterização estrutural e avaliação citotóxica de orto-(alquiltio)acetanilidas. Deste modo, foram sintetizadas, e totalmente caracterizadas por RMN de 1H e 13C, FTIR e HRMS (FAB), cinco novas orto-(alquiltio)acetanilidas. Adicionalmente, foi avaliada a citotoxicidade in vitro destas alquiltioacetanilidas e de um conjunto de outras orto-(alquilcalcogénio)acetanilidas relacionadas, num total de vinte acetanilidas representativas, nas linhas celulares de células epiteliais humanas do cancro da mama (MCF-7), células do adenocarcinoma da próstata (LNCaP) e fibroblastos normais da derme humana (NHDF). Dos resultados obtidos, realça-se a atividade antiproliferativa potente das benzil-, (2-metil)benzil- e hexil-acetanilidas nas linhas celulares cancerígenas, com destaque nas LNCaP.
The work presented in this document comes from the knowledge acquired during the three parts developed at the final stage of the Integrated Master in Pharmaceutical Sciences. Hence, this internship report is divided into three chapters, each one resultant from one of the parts mentioned above. The first chapter deals with the professional experience acquired during the internship conducted in the Taborda Pharmacy, located in Fundão city. It describes all knowledge and skills acquired, taking in consideration the Good Practices in Community Pharmacy and the Pharmaceutical activity regulator laws. The second chapter describes all the experience acquired during the internship developed at Local Health Unit Sousa Martins Hospital in Guarda city. Therefore, the activities undertaken during this internship part are described within this report regarding the role played by hospital pharmacist, as described in the actual legislation. In the third chapter, the research activities developed at “Organic Synthesis Laboratory” located on Chemical Department of Faculty of Sciences and at Health Sciences Research Centre are reported. The aim of this work was the synthesis, structural characterization and cytotoxic evaluation of orto-(alkylthio)acetanilides. Therefore, five new orto-(alkylthio)acetanilides were synthetized and fully characterized by 1H e 13C, FTIR e HRMS (FAB). Moreover, the in vitro cytotoxic evaluation of these thioacetanilides and a set of others related orto-(alkylcalcogens)acetanilides in a total of twenty compounds against human breast (MCF-7) and prostate (LNCaP) cancer cell lines as well as normal human dermal fibroblasts (NHDF) were also evaluated. Interestingly a selective antiproliferative activity was observed for cancerous cells with proeminence to LNCaP within the most potent O- and/or N-benzylic and -hexyl acetanilides.
The work presented in this document comes from the knowledge acquired during the three parts developed at the final stage of the Integrated Master in Pharmaceutical Sciences. Hence, this internship report is divided into three chapters, each one resultant from one of the parts mentioned above. The first chapter deals with the professional experience acquired during the internship conducted in the Taborda Pharmacy, located in Fundão city. It describes all knowledge and skills acquired, taking in consideration the Good Practices in Community Pharmacy and the Pharmaceutical activity regulator laws. The second chapter describes all the experience acquired during the internship developed at Local Health Unit Sousa Martins Hospital in Guarda city. Therefore, the activities undertaken during this internship part are described within this report regarding the role played by hospital pharmacist, as described in the actual legislation. In the third chapter, the research activities developed at “Organic Synthesis Laboratory” located on Chemical Department of Faculty of Sciences and at Health Sciences Research Centre are reported. The aim of this work was the synthesis, structural characterization and cytotoxic evaluation of orto-(alkylthio)acetanilides. Therefore, five new orto-(alkylthio)acetanilides were synthetized and fully characterized by 1H e 13C, FTIR e HRMS (FAB). Moreover, the in vitro cytotoxic evaluation of these thioacetanilides and a set of others related orto-(alkylcalcogens)acetanilides in a total of twenty compounds against human breast (MCF-7) and prostate (LNCaP) cancer cell lines as well as normal human dermal fibroblasts (NHDF) were also evaluated. Interestingly a selective antiproliferative activity was observed for cancerous cells with proeminence to LNCaP within the most potent O- and/or N-benzylic and -hexyl acetanilides.
Description
Keywords
Análogos da Nimesulida Avaliação Citotóxica Farmácia Comunitária Farmácia Hospitalar Linhas Celulares Nhdf Mcf-7 e Lncap Orto-(Alquilcalcogénio)Acetanilidas Orto-(Alquiltio)Acetanilidas Síntese e Caracterização