| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| Tese - 1ª parte | 1.47 MB | Adobe PDF | ||
| Tese - 2ª parte | 3.8 MB | Adobe PDF |
Advisor(s)
Abstract(s)
O presente relatório de estágio é constituído por duas partes, sendo a Parte I referente à investigação experimental, e a Parte II relativa ao estágio desenvolvido ao nível da farmácia comunitária.
O principal objetivo da Parte I é avaliar a atividade anti-inflamatória de extratos metanólicos de diferentes partes da planta Pterospartum tridentatum, nomeadamente dos caules, folhas e flores. Os extratos brutos foram obtidos por extração com soxhlet e avaliados quanto à atividade anti-inflamatória. Esta atividade foi avaliada através do método que verifica a eventual ação inibitória dos extratos sobre a enzima 5-lipoxigenase e consequente inibição da formação de 5-HETE (ácido 5-hidroxieicosatetraenóico), sendo esta reação seguida espectrofotometricamente a 234 nm. Esta reação foi também seguida através de um ensaio colorimétrico a 620 nm (ensaio FOX), que pretende avaliar a atividade enzimática da enzima 5-lipoxigenase por formação de um complexo entre Fe3+ e o laranja xilenol que absorve na região do visível, mimetizando a hidroperoxidação lipídica que ocorre no organismo durante o processo inflamatório. Para este efeito estas metodologias foram implementadas em laboratório.
A Parte II tem como principal objetivo abordar sucintamente as etapas da minha formação ao nível do funcionamento da farmácia comunitária, bem como o papel do farmacêutico enquanto diretor técnico e como farmacêutico adjunto, enquanto profissional de saúde especialista do medicamento e agente de saúde pública.
This report consists of two parts, Part I concerning the experimental investigation, and Part II on the stage developed in community pharmacy. The main purpose of Part I was to evaluate the anti-inflammatory activity of methanolic extracts from different parts of the shrub Pterospatum tridentatum, particularly the extracts of the stems, leaves and flowers. The crude extracts were obtained by soxhlet extraction and the anti-inflammatory activity was evaluated. This activity was evaluated using the method that verifies the potential inhibition of 5-lipoxygenase enzyme to create 5- HETE by the extracts and the reaction was followed by spectrophotometry at 234 nm. A colorimetric assay was also carried out (FOX assay) to evaluate the enzymatic activity of 5- lipoxygenase enzyme by forming a complex between Fe3+ and orange xylenol which absorbs in the visible region, mimicking the lipid hydroperoxidation that occurs during the inflammatory process. For this purpose, these methods were implemented in the laboratory. Part II aims to describe the stages of my formation in community pharmacy, as well as the role of the pharmacist as technical director and as assistant pharmacist, and as a specialist and public health worker.
This report consists of two parts, Part I concerning the experimental investigation, and Part II on the stage developed in community pharmacy. The main purpose of Part I was to evaluate the anti-inflammatory activity of methanolic extracts from different parts of the shrub Pterospatum tridentatum, particularly the extracts of the stems, leaves and flowers. The crude extracts were obtained by soxhlet extraction and the anti-inflammatory activity was evaluated. This activity was evaluated using the method that verifies the potential inhibition of 5-lipoxygenase enzyme to create 5- HETE by the extracts and the reaction was followed by spectrophotometry at 234 nm. A colorimetric assay was also carried out (FOX assay) to evaluate the enzymatic activity of 5- lipoxygenase enzyme by forming a complex between Fe3+ and orange xylenol which absorbs in the visible region, mimicking the lipid hydroperoxidation that occurs during the inflammatory process. For this purpose, these methods were implemented in the laboratory. Part II aims to describe the stages of my formation in community pharmacy, as well as the role of the pharmacist as technical director and as assistant pharmacist, and as a specialist and public health worker.
Description
Keywords
Pterospartum tridentatum - Extratos metanólicos - Actividade anti-inflamatória Flavonóides Ensaio espectrofotométrico Farmácia comunitária Farmacêutico - Utente - Medicamento
Pedagogical Context
Citation
Publisher
Universidade da Beira Interior