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Abstract(s)
Pseudomonas aeruginosa é uma bactéria com a capacidade de causar graves problemas a
nível de saúde pública devido à sua grande versatilidade genética, à produção de diversos
fatores de virulência, à resistência antibiótica e à formação de biofilmes. Contudo, a
obrigatoriedade da análise deste microrganismo em águas de consumo humano não está
presente em nenhum Decreto-Lei. Como tal, surge a necessidade de implementar métodos
que analisem a presença desta espécie nas águas de consumo humano devido aos perigos
associados.
No decorrer deste estágio curricular, na ALS Life Sciences, foi implementado e estudado o
método “Deteção e enumeração de Pseudomonas aeruginosa em águas de consumo
humano” segundo a norma ISO 16266:2006 - Qualidade da água - Deteção e enumeração
de Pseudomonas aeruginosa - método por filtração de membrana. De acordo com a norma
ISO 16266:2006 este ensaio é dividido em 3 etapas fundamentais: filtração, enumeração e
confirmação. Para tal, foram recolhidas diversas amostras de água de consumo humano de
diversos locais e após contaminadas com diversas estirpes de Pseudomonas aeruginosa
foram filtradas, enumeradas e confirmadas.
Na implementação do método foram analisados diversos parâmetros, desde o controlo de
qualidade do meio de cultura, as caraterísticas de performance do método, que incluem a
sensibilidade, a especificidade, a taxa de falsos positivos, a taxa de falsos negativos, a
seletividade, a eficiência, a repetibilidade e a incerteza. Para além disso, foi avaliada também
a precisão e a exatidão.
Tendo em consideração os resultados obtidos nos ensaios realizados, verificou-se que o
método foi bem implementado, sendo adequado aos fins a que se destina e estando o
laboratório de microbiologia de Castelo Branco da ALS Life Sciences apto para efetuar esta
análise.
Pseudomonas aeruginosa is a bacterium with the capacity to cause serious public health problems due to its great genetic versatility, the production of various virulence factors, antibiotic resistance and the formation of biofilms. However, the mandatory analysis of this microorganism in water for human consumption is not included in any Decree-Law. As such, there is a need to implement methods that analyze the presence of this species in water for human consumption due to the associated dangers. During this curricular internship, at ALS Life Sciences, the method “Detection and enumeration of Pseudomonas aeruginosa in water for human consumption” was implemented and studied according to the ISO standard 16266:2006 - Water quality - Detection and enumeration of Pseudomonas aeruginosa - method by membrane filtration. According to the ISO standard 16266:2006, this test is divided into 3 fundamental steps: filtration, enumeration and confirmation. To do this, several samples of water for human consumption were collected from different locations and, after being contaminated with different strains of Pseudomonas aeruginosa, they were filtered, enumerated and confirmed. When applying the method, several parameters were analyzed, from quality control of the culture medium, as well as performance characteristics of the method, which include sensitivity, specificity, false positive rate, false negative rate, selectivity, efficiency, repeatability and uncertainty. In addition, precision and accuracy were also evaluated. Considering the results obtained in the tests carried out, it was verified that the method was well implemented, being suitable for the purposes for which it was intended and the ALS Life Sciences microbiology laboratory in Castelo Branco is able to carry out this analysis.
Pseudomonas aeruginosa is a bacterium with the capacity to cause serious public health problems due to its great genetic versatility, the production of various virulence factors, antibiotic resistance and the formation of biofilms. However, the mandatory analysis of this microorganism in water for human consumption is not included in any Decree-Law. As such, there is a need to implement methods that analyze the presence of this species in water for human consumption due to the associated dangers. During this curricular internship, at ALS Life Sciences, the method “Detection and enumeration of Pseudomonas aeruginosa in water for human consumption” was implemented and studied according to the ISO standard 16266:2006 - Water quality - Detection and enumeration of Pseudomonas aeruginosa - method by membrane filtration. According to the ISO standard 16266:2006, this test is divided into 3 fundamental steps: filtration, enumeration and confirmation. To do this, several samples of water for human consumption were collected from different locations and, after being contaminated with different strains of Pseudomonas aeruginosa, they were filtered, enumerated and confirmed. When applying the method, several parameters were analyzed, from quality control of the culture medium, as well as performance characteristics of the method, which include sensitivity, specificity, false positive rate, false negative rate, selectivity, efficiency, repeatability and uncertainty. In addition, precision and accuracy were also evaluated. Considering the results obtained in the tests carried out, it was verified that the method was well implemented, being suitable for the purposes for which it was intended and the ALS Life Sciences microbiology laboratory in Castelo Branco is able to carry out this analysis.
Description
Keywords
Iso Pseudomonas Aeruginosa Qualidade da Água Saúde