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Abstract(s)
A presente dissertação insere-se na unidade curricular Estágio do Mestrado Integrado
em Ciências Farmacêuticas da Universidade da Beira Interior. Esta divide-se em duas
partes, a de investigação e a do estágio em farmácia comunitária.
O primeiro capítulo diz respeito ao trabalho de investigação que foi realizado no Centro
de Investigação em Ciências da Saúde da Universidade da Beira Interior. O estudo
realizado teve como objetivo a avaliação da atividade anti-Candida de diferentes óleos
essenciais e águas minerais naturais com posterior desenvolvimento de uma formulação
em spray para o tratamento da candidose oral.
A candidose oral é uma condição causada pelo fungo Candida albicans, que faz parte da
flora comensal humana. Quando a imunidade do organismo está fragilizada, este
microrganismo pode crescer em excesso e causar infeções, como a candidose oral. Para
a escolha dos ingredientes ativos a utilizar numa formulação que pudesse tratar esta
doença, foi determinada a concentração mínima inibitória de diferentes óleos essenciais,
Origanum vulgare, Thymus zygis e Thymus mastichina e da água mineral natural de S.
Pedro do Sul, recorrendo ao método de microdiluição em placas. As concentrações
mínimas inibitórias obtidas para cada óleo essencial foram respetivamente 0,31% (v/v),
0,63% (v/v) e 2,50% (v/v). Foram também realizados ensaios cruzados de forma a avaliar
a interação entre cada óleo essencial e a água mineral natural. Para a realização da
formulação foi utilizado o óleo essencial que apresentava uma maior capacidade de
inibição do crescimento de Candida albicans, o óleo essencial de Origanum vulgare,
juntamente com a água mineral natural de S. Pedro do Sul, um agente tensioativo, um
conservante e um booster de conservante. A formulação protótipo apresentou atividade
relevante que foi avaliada, apresentando uma concentração mínima inibitória de 6,25%
(v/v), o que mostra o potencial que estes ingredientes têm para serem utilizados em
futuras formulações.
O segundo capítulo desta dissertação refere-se à experiência profissionalizante em
farmácia comunitária, obtida através do estágio realizado na Farmácia Barbosa,
localizada em Vila Nova de Anha, que decorreu de 6 de fevereiro a 24 de junho de 2023.
Nesta secção estarão apontadas todas as atividades realizadas, assim como os
conhecimentos adquiridos ao longo de todo o estágio curricular.
This dissertation is part of the Internship curricular unit of the Integrated Master's Degree in Pharmaceutical Sciences at the University of Beira Interior. This is divided into two parts, research and internship in community pharmacy. The first chapter refers to the research work developed at the Health Sciences Research Center of the University of Beira Interior. The study aimed to evaluate the anti-Candida activity of essential oils and natural mineral waters and then develop a spray formulation for the treatment of oral candidosis. Oral candidosis is a condition caused by the fungus Candida albicans, which is part of the human commensal flora. When the body's immunity is compromised, this microorganism can overgrow and cause infections, such as oral candidiasis. In order to select the active ingredients to be used in a formulation that could treat this disease, the minimum inhibitory concentration of different essential oils, Origanum vulgare, Thymus zygis and Thymus mastichina and S. Pedro do Sul natural mineral water, was determined, using the plate microdilution method. The minimum inhibitory concentrations obtained for each essential oil were 0.31% (v/v), 0.63% (v/v) and 2.50% (v/v), respectively. Cross-over tests were also carried out to evaluate the interaction between each essential oil and the thermal water. To create the formulation, the essential oil that had a greater capacity to inhibit the growth of Candida albicans, the Origanum vulgare essential oil, was used, together with the S. Pedro do Sul natural mineral water, a surfactant, a preservative and a preservative booster. The prototype formulation activity was evaluated, showing a great capacity to inhibit Candida albicans growth, with a minimum inhibitory concentration of 6.25% (v/v), suggesting the potential that these ingredients have to be used in future formulations. The second chapter of this dissertation refers to the professional experience in community pharmacy, obtained through the internship performed at Farmácia Barbosa, located in Vila Nova de Anha, which took place from February 6th to June 24th, 2023. This section will highlight all the carried-out activities, as well as the knowledge acquired throughout the curricular internship.
This dissertation is part of the Internship curricular unit of the Integrated Master's Degree in Pharmaceutical Sciences at the University of Beira Interior. This is divided into two parts, research and internship in community pharmacy. The first chapter refers to the research work developed at the Health Sciences Research Center of the University of Beira Interior. The study aimed to evaluate the anti-Candida activity of essential oils and natural mineral waters and then develop a spray formulation for the treatment of oral candidosis. Oral candidosis is a condition caused by the fungus Candida albicans, which is part of the human commensal flora. When the body's immunity is compromised, this microorganism can overgrow and cause infections, such as oral candidiasis. In order to select the active ingredients to be used in a formulation that could treat this disease, the minimum inhibitory concentration of different essential oils, Origanum vulgare, Thymus zygis and Thymus mastichina and S. Pedro do Sul natural mineral water, was determined, using the plate microdilution method. The minimum inhibitory concentrations obtained for each essential oil were 0.31% (v/v), 0.63% (v/v) and 2.50% (v/v), respectively. Cross-over tests were also carried out to evaluate the interaction between each essential oil and the thermal water. To create the formulation, the essential oil that had a greater capacity to inhibit the growth of Candida albicans, the Origanum vulgare essential oil, was used, together with the S. Pedro do Sul natural mineral water, a surfactant, a preservative and a preservative booster. The prototype formulation activity was evaluated, showing a great capacity to inhibit Candida albicans growth, with a minimum inhibitory concentration of 6.25% (v/v), suggesting the potential that these ingredients have to be used in future formulations. The second chapter of this dissertation refers to the professional experience in community pharmacy, obtained through the internship performed at Farmácia Barbosa, located in Vila Nova de Anha, which took place from February 6th to June 24th, 2023. This section will highlight all the carried-out activities, as well as the knowledge acquired throughout the curricular internship.
Description
Keywords
Origanum Vulgare Água Mineral Natural Atividade Antimicrobiana Candida Albicans Farmácia
Comunitária Formulação Óleo Essencial Thymus Mastichina Thymus Zygis