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Abstract(s)
O presente trabalho surge no âmbito da conclusão do Mestrado Integrado em Ciências
Farmacêuticas e descreve a minha experiência profissionalizante nas vertentes de
investigação e farmácia comunitária. O trabalho apresentado encontra-se dividido em dois
capítulos.
O Capítulo I abordará a experiência profissionalizante adquirida em Farmácia Comunitária
durante o estágio realizado na Farmácia Taborda (FT). Este descreve os conhecimentos e
competências adquiridas tendo em conta a legislação vigente bem como as Boas Praticas
em Farmácia.
Este capítulo consiste numa breve descrição da farmácia e do seu funcionamento, das
atividades desenvolvidas, e dos conhecimentos adquiridos e postos em prática ao longo do
estágio. Serão ainda abordados outros temas, nomeadamente, a interação farmacêuticodoente-medicamento e os cuidados de saúde prestados, a dispensa de medicamentos
sujeitos e não sujeitos a receita médica, a atitude do farmacêutico no ato da dispensa, a
indicação farmacêutica nos casos de automedicação, entre outros, que são essenciais à boa
prática da profissão farmacêutica
O Capítulo II refere-se ao trabalho de investigação desenvolvido. Foi realizada uma revisão
sistemática da literatura sobre o tema “Técnica de amostragem Dried Blood Spots (DBS)
para fins bioanalíticos”, com objetivo de analisar a aplicabilidade dos DBS em toxicologia.
DBS consiste numa técnica de amostragem, mas também de preparação de amostras de
sangue.
Os DBS são um procedimento de preparação de amostras simples e menos oneroso tendo
em conta que o papel de filtro permite a retenção de vários componentes da matriz
biológica, resultando em maior facilidade do procedimento de extração.
Além disso, como uma técnica de amostragem, o DBS é rápido e pouco invasivo e os níveis
dos analitos avaliados são possíveis correlacionar com sintomas, ao contrário da urina.
Adicionalmente, os DBS requerem uma quantidade mínima de sangue; permite a análise de
uma enorme variedade de compostos como ácidos nucleicos, proteínas, lípidos ou pequenas
moléculas orgânicas e não orgânicas, quando comparados com os testes sanguíneos
convencionais, proporciona uma melhoria pratica, clínica e financeira.
Conclui-se assim que, em situações cujo tempo de resposta rápido não se constituiu uma condição sine qua non, a utilização dos DBS, quer como técnica de amostragem, quer como
procedimento de preparação das amostras, configura-se como útil para as análises de
triagem, identificação e/ou quantificação de diversos analitos.
This work is part of the Integrated Master's Degree in Pharmaceutical Sciences’ conclusion and describes the professional experience in the fields of research and community pharmacy. The present work is divided into two chapters. Chapter I will approach the professional experience acquired in community pharmacy during the internship held at Farmácia Taborda (FT). This will describe the knowledge and skills acquired regarding the current legislation as well as the Good Practices in Pharmacy. This chapter consists of a brief description of the pharmacy and its functioning, the developed activities, the knowledge acquired and put into practice throughout the internship. Several other topics are also addressed, namely, the pharmaceutical-patientdrug interaction and the health care provided, non-prescription drugs pharmaceutical recommendation, the pharmaceutical indication in cases of self-medication, among others, which are essential for a good practice of the pharmaceutical work. Chapter II refers to the research work developed. A systematic review of the literature was performed on the subject ‘Sampling Technique Dried Blood Spots (DBS) for bioanalytical purposes’, with the aim to evaluate the applicability of DBS in toxicology. DBS consists of a sampling technique but can also be used as a blood sample preparation procedure. DBS are a simple and less expensive sample preparation procedures, considering that the filter paper allows the retention of several analytes of the biological matrix and the extraction procedure is easier. Moreover, as a sampling technique, DBS is little invasive and rapid, and the levels of analytes measured are possible to correlate symptoms, unlike for urine. Additionally, DBS requires a minimum amount of blood; allows the testing of a wide range of compounds as nucleic acids, proteins, lipids or small organic and non-organic molecules and, when compared to conventional blood tests, provide a practical, clinical and financial improvement. It was possible to concluded that in situations that a rapid response did not constitute a sine qua non condition, the use of DBS, either as a sampling technique or as a sample preparation procedure, is useful for screening, identification and/or quantification of many analytes.
This work is part of the Integrated Master's Degree in Pharmaceutical Sciences’ conclusion and describes the professional experience in the fields of research and community pharmacy. The present work is divided into two chapters. Chapter I will approach the professional experience acquired in community pharmacy during the internship held at Farmácia Taborda (FT). This will describe the knowledge and skills acquired regarding the current legislation as well as the Good Practices in Pharmacy. This chapter consists of a brief description of the pharmacy and its functioning, the developed activities, the knowledge acquired and put into practice throughout the internship. Several other topics are also addressed, namely, the pharmaceutical-patientdrug interaction and the health care provided, non-prescription drugs pharmaceutical recommendation, the pharmaceutical indication in cases of self-medication, among others, which are essential for a good practice of the pharmaceutical work. Chapter II refers to the research work developed. A systematic review of the literature was performed on the subject ‘Sampling Technique Dried Blood Spots (DBS) for bioanalytical purposes’, with the aim to evaluate the applicability of DBS in toxicology. DBS consists of a sampling technique but can also be used as a blood sample preparation procedure. DBS are a simple and less expensive sample preparation procedures, considering that the filter paper allows the retention of several analytes of the biological matrix and the extraction procedure is easier. Moreover, as a sampling technique, DBS is little invasive and rapid, and the levels of analytes measured are possible to correlate symptoms, unlike for urine. Additionally, DBS requires a minimum amount of blood; allows the testing of a wide range of compounds as nucleic acids, proteins, lipids or small organic and non-organic molecules and, when compared to conventional blood tests, provide a practical, clinical and financial improvement. It was possible to concluded that in situations that a rapid response did not constitute a sine qua non condition, the use of DBS, either as a sampling technique or as a sample preparation procedure, is useful for screening, identification and/or quantification of many analytes.
Description
Keywords
Dried Blood Spots Farmácia Comunitária Toxicologia