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Advisor(s)
Abstract(s)
Following the intensification of agriculture and the promotion of agro-chemicals in low and
middle income countries, acute pesticide poisoning has become a major public health problem
with more than 300,000 deaths each year around the world.
The easy availability of highly toxic pesticides in the homes of farming communities has made
pesticides a preferred choice for suicide with an extremely high case fatality. In fact, the World
Health Organization (WHO) indicates that there may be 1 million serious unintentional
poisonings each year and in addition 2 million people hospitalized for suicide attempts with
pesticides.
The goal of this work was the detection and quantification of eight organophosphorous
pesticides in blood samples using solid phase extraction and gas chromatography-mass
spectrometry.
The studied analytes were omethoate, dimethoate, diazinon, chlorpyrifos, parathion,
clorfenvinphos, quinalphos and azinphos. Ethion was used as internal standard (IS).
The analytes and IS were extracted by solid-phase extraction using Oasis® HLB extraction
cartridges, and the extracts were analyzed by gas chromatography-electron ionisation-mass
spectrometry (GC/EI-MS). Calibration curves were established using a weighed linear
calibration model (except for omethoate, for which a power regression was used) between 0.05
and 25.00 μg/mL. The correlation coefficients were higher than 0.991. Precision (intraday and
intermediate) and accuracy were in conformity with the criteria normally accepted in
bioanalytical method validation. Limits of quantification were 50 ng/mL for all compounds,
except for ometoathe, for which 100 ng/mL were obtained. Because of its simplicity and speed, the proposed method can be applied in the determination of
these compounds in post-mortem blood samples, and is suitable for application in toxicology
routine analysis.
Description
Keywords
Pesticidas organofosforados Analitos Cromatografia gasosa