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Abstract(s)
O presente relatório visa a última etapa para a obtenção do grau de Mestre em Ciências
Farmacêuticas, sendo que este se encontra dividido em três capítulos. O primeiro
capítulo diz respeito ao trabalho de investigação realizado no Centro de Investigação
em Ciências da Saúde (CICS-UBI), no qual se otimizou uma técnica para a préconcentração de canabinóides a partir de amostras de cabelo com recurso à
microextração em seringa empacotada (MEPS) sob orientação do Doutor Tiago
Alexandre Pires Rosado e coorientação da Professora Doutora Maria Eugenia Gallardo
Alba. A canábis é a droga de abuso mais consumida no mundo. Deste modo é relevante
desenvolver métodos analíticos rápidos e sensíveis para a sua determinação. A amostra
de cabelo é vantajosa porque apresenta uma ampla janela de deteção, uma colheita
menos invasiva e é de difícil adulteração. Os compostos alvo de estudo foram o
canabidiol (CBD), delta-9-tetrahidrocanabinol (THC), canabinol (CBN), 11-hidroxidelta-9-tetrahidrocanabinol (THC-OH) e 11-Nor-9-carboxi-delta-9-tetrahidrocanabinol
(THC-COOH). A MEPS é vantajosa pela reutilização do sorbente e os pequenos
volumes usados. Para a otimização do procedimento, foi realizado um desenho
experimental onde se verificou que o fator com maior influência na resposta foi o
número de aspirações da amostra. Seguidamente efetuou-se um estudo univariado
deste fator e outro na lavagem para perceber se o metanol afetava a recuperação dos
compostos. As condições otimizadas foram: acondicionamento do sorbente com
metanol e 2% de ácido fórmico (2 vezes 250µL); aspiração da amostra (15 vezes 150µL);
lavagem com 3,36% de ácido fórmico (50µL) e 5% de metanol (50µL) e a eluição com
2,36% de hidróxido de amónia em metanol (6 vezes 100µL). O segundo capítulo
assenta na experiência do estágio em Farmácia Hospitalar, realizado no Hospital
Universitário Cova da Beira, na Covilhã, sob supervisão da Professora Doutora Olímpia
Fonseca. Finalmente, o terceiro capítulo descreve a experiência obtida durante o
estágio em Farmácia Comunitária, realizado na Farmácia São Cosme, na cidade da
Covilhã, sob supervisão do Dr. Carlos Tavares.
This report is the last step towards obtaining the Master's degree in Pharmaceutical Sciences, and is divided into three chapters. The first chapter concerns the research work carried out at the Health Sciences Research Center (CICS-UBI), in which we optimized a technique to pre-concentrate cannabinoids from hair samples using microextraction by packed sorbent (MEPS) under the guidance of Doctor Tiago Alexandre Pires Rosado and co-supervision of Professor Doctor Maria Eugenia Gallardo Alba. Cannabis is the most widely consumed drug of abuse in the world. Thus, it is relevant to develop rapid and sensitive analytical methods for its determination. The hair sample is advantageous because it presents a wide detection window, a less invasive collection and is difficult to tamper with. The target compounds of the study were cannabidiol (CBD), delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), 11- hydroxy-delta-9-tetrahydrocannabinol (THC-OH) and 11-Nor-9-carboxy-delta-9- tetrahydrocannabinol (THC-COOH). MEPS is advantageous because of the reuse of the sorbent and the small volumes required. To optimize the procedure, an experimental design was carried out where it was found that the factor with the greatest influence on the response was the number of sample draw/eject cycles. A univariate study was then performed on this factor and another on the washing to understand if methanol affected the recovery of the compounds. The optimized conditions were: sorbent conditioning with methanol and 2% formic acid (2 times 250µL); sample draw/eject (15 times 150µL); washing with 3.36% formic acid (50µL) and 5% methanol (50µL); and elution with 2.36% ammonium hydroxide in methanol (6 times 100µL). The second chapter is based on the experience of the internship in Hospital Pharmacy, carried out at Cova da Beira University Hospital, in Covilhã, under the supervision of Professor Doctor Olímpia Fonseca. Finally, the third chapter describes the experience obtained during the internship in Community Pharmacy, carried out at Pharmacy São Cosme, in the city of Covilhã, under the supervision of Doctor Carlos Tavares.
This report is the last step towards obtaining the Master's degree in Pharmaceutical Sciences, and is divided into three chapters. The first chapter concerns the research work carried out at the Health Sciences Research Center (CICS-UBI), in which we optimized a technique to pre-concentrate cannabinoids from hair samples using microextraction by packed sorbent (MEPS) under the guidance of Doctor Tiago Alexandre Pires Rosado and co-supervision of Professor Doctor Maria Eugenia Gallardo Alba. Cannabis is the most widely consumed drug of abuse in the world. Thus, it is relevant to develop rapid and sensitive analytical methods for its determination. The hair sample is advantageous because it presents a wide detection window, a less invasive collection and is difficult to tamper with. The target compounds of the study were cannabidiol (CBD), delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), 11- hydroxy-delta-9-tetrahydrocannabinol (THC-OH) and 11-Nor-9-carboxy-delta-9- tetrahydrocannabinol (THC-COOH). MEPS is advantageous because of the reuse of the sorbent and the small volumes required. To optimize the procedure, an experimental design was carried out where it was found that the factor with the greatest influence on the response was the number of sample draw/eject cycles. A univariate study was then performed on this factor and another on the washing to understand if methanol affected the recovery of the compounds. The optimized conditions were: sorbent conditioning with methanol and 2% formic acid (2 times 250µL); sample draw/eject (15 times 150µL); washing with 3.36% formic acid (50µL) and 5% methanol (50µL); and elution with 2.36% ammonium hydroxide in methanol (6 times 100µL). The second chapter is based on the experience of the internship in Hospital Pharmacy, carried out at Cova da Beira University Hospital, in Covilhã, under the supervision of Professor Doctor Olímpia Fonseca. Finally, the third chapter describes the experience obtained during the internship in Community Pharmacy, carried out at Pharmacy São Cosme, in the city of Covilhã, under the supervision of Doctor Carlos Tavares.
Description
Keywords
Cabelo Canabinóides Farmácia Comunitária Farmácia Hospitalar Meps