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Abstract(s)
Introdução: Atualmente o Cancro Colorretal é uma das neoplasias mais diagnosticadas
nos países desenvolvidos. Na Europa, acomete 17 em cada 100 000 habitantes. Apresenta
baixa incidência abaixo dos 50 anos de idade e é mais frequente em homens do que
mulheres. Os principais fatores de risco para desenvolver esta neoplasia são idade
avançada e história familiar.
O fator de prognóstico mais importante desta doença é o estadiamento, pois a escolha da
abordagem terapêutica recai principalmente nesta avaliação. Para auxiliar na avaliação do
prognóstico dos pacientes, numerosos marcadores genéticos e bioquímicos têm vindo a
ser sugeridos, no entanto, ainda não forneceram provas robustas para serem aplicados na
prática clínica.
Este estudo teve por objetivo identificar a presença ou ausência do marcador de
proliferação celular, Ki-67, em adenocarcinomas colorretais e verificar se este é um fator
de prognóstico, ou se têm correlação com outras variáveis de interesse clínico, de forma a
estudar o possível interesse do uso deste marcador em combinação com outras avaliações
anatomopatológicas.
Objetivos: Verificar se o marcador de proliferação celular Ki-67 é preditivo de
estadiamento, localização, diferenciação celular, prognóstico, sexo e idade em pacientes
com adenocarcinoma colorretal.
Métodos: Neste estudo retrospetivo foram estudados 30 pacientes com Adenocarcinoma
Colorretal, submetidos a ressecção cirúrgica com intenção curativa entre 1 de janeiro de
2004 e 1 de janeiro de 2007 no Centro Hospitalar Universitário Cova da Beira, que
posteriormente foram sujeitos a um follow-up pós-cirúrgico nos 10 anos seguintes.
As amostras recolhidas foram processadas no laboratório de Anatomia Patológica da
instituição anteriormente mencionada, onde decorreu a classificação histológica e
avaliação da expressão imunohistoquímica do Ki-67. O anticorpo MIB-1, dirigido ao
antigénio Ki-67 humano, foi usado para este processo de marcação imunohistoquímica.
Os dados recolhidos foram analisados estatisticamente recorrendo ao Teste Exato de
Fisher, onde utilizou-se um intervalo de confiança de 95% e foi considerado
estatisticamente significativo um valor p <0.05. Foi também analisado o coeficiente de
associação entre as variáveis, V de Cramer. Resultados: Foi encontrada correlação estatisticamente significativa (p<0.05) entre a
marcação celular com o marcador de proliferação celular Ki-67 e duas das variáveis
estudadas: “prognóstico” e “estadiamento”. Não foram obtidas relações estatisticamente
significativas com outras variáveis estudadas.
Conclusão: Os resultados encontrados neste trabalho estão em sintonia com os
resultados apresentados em outros estudos sobre o papel da proteína Ki-67, onde se
conclui que esta está associada ao prognóstico e estadiamento de adenocarcinoma do
cólon.
Introduction: Nowadays Colorectal Cancer is one of the most diagnosed neoplasms in developed countries. In Europe it affects 17 out of every 100 000 inhabitants. It is infrequent below the age of 50 and affects more men than women. The main risk factors for developing this neoplasm are advanced age and family history of the disease. The most important factor of prognosis is the staging of the disease, as the choice of therapeutic approach falls mainly on this assessment. To assist in the evaluation of the prognosis of patients numerous genetic and biochemical markers have been suggested, but they have not yet provided robust evidence to be applied in clinical practice. This study aims to identify the presence or absence of the cell proliferation marker, Ki-67, in colorectal adenocarcinoma and to verify if it is a prognostic factor, or if it has correlation with other variables of clinical interest. Objectives: To verify if the Ki-67 cell proliferation marker is predictive of staging, location, cell differentiation of prognosis, age and gender in patients with colorectal adenocarcinoma. Methods: In this retrospective study, 30 patients with colorectal adenocarcinoma who underwent surgical resection with curative intention between January 1th 2004 and January 1th 2007 at the University of Beira Interior Hospital Center, who underwent postmedical monitoring in the following 10 years. The samples collected were processed in the Laboratory of Pathology of the mentioned institution, where the histological classification and evaluation of the immunohistochemical expression of Ki-67 took place. The MIB-1 antibody, directed to the human Ki-67 antigen was used for this process of immunohistochemestry marking. The collected data were analyzed statistically using Fisher´s Exact Test, where a 95% confidence interval was used and p value < 0.05 was considered statistically significant. The coefficient of association between variables was analyzed with Cramer´s V. Results: Statistically significant correlation (p<0.05) was found between the celular proliferation marker Ki-67 and two of the studied variables: “prognosis” and “staging”. No statistically significant relationships with other studied variables were obtained. Conclusion: The results found in this study are in line with the results presented in other studies about Ki-67 role in colon adenocarcinomas, witch conclude that it is associated with the prognosis and staging of this neoplasia.
Introduction: Nowadays Colorectal Cancer is one of the most diagnosed neoplasms in developed countries. In Europe it affects 17 out of every 100 000 inhabitants. It is infrequent below the age of 50 and affects more men than women. The main risk factors for developing this neoplasm are advanced age and family history of the disease. The most important factor of prognosis is the staging of the disease, as the choice of therapeutic approach falls mainly on this assessment. To assist in the evaluation of the prognosis of patients numerous genetic and biochemical markers have been suggested, but they have not yet provided robust evidence to be applied in clinical practice. This study aims to identify the presence or absence of the cell proliferation marker, Ki-67, in colorectal adenocarcinoma and to verify if it is a prognostic factor, or if it has correlation with other variables of clinical interest. Objectives: To verify if the Ki-67 cell proliferation marker is predictive of staging, location, cell differentiation of prognosis, age and gender in patients with colorectal adenocarcinoma. Methods: In this retrospective study, 30 patients with colorectal adenocarcinoma who underwent surgical resection with curative intention between January 1th 2004 and January 1th 2007 at the University of Beira Interior Hospital Center, who underwent postmedical monitoring in the following 10 years. The samples collected were processed in the Laboratory of Pathology of the mentioned institution, where the histological classification and evaluation of the immunohistochemical expression of Ki-67 took place. The MIB-1 antibody, directed to the human Ki-67 antigen was used for this process of immunohistochemestry marking. The collected data were analyzed statistically using Fisher´s Exact Test, where a 95% confidence interval was used and p value < 0.05 was considered statistically significant. The coefficient of association between variables was analyzed with Cramer´s V. Results: Statistically significant correlation (p<0.05) was found between the celular proliferation marker Ki-67 and two of the studied variables: “prognosis” and “staging”. No statistically significant relationships with other studied variables were obtained. Conclusion: The results found in this study are in line with the results presented in other studies about Ki-67 role in colon adenocarcinomas, witch conclude that it is associated with the prognosis and staging of this neoplasia.
Description
Keywords
Diferenciação Histológica Adenocarcinoma do Cólon Cancro Colorretal Idade Imunohistoquímica Ki-67 Prognóstico Sexo