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Abstract(s)
Os estrogĂ©nios sĂŁo hormonas sexuais esteroides que, nas mulheres, sĂŁo responsĂĄveis pelo desenvolvimento do sistema reprodutivo. Existem vĂĄrios tipos de estrogĂ©nios com diferentes funçÔes. A estrona (E1) Ă© considerada um estrogĂ©nio fraco e existe numa concentração inferior Ă do estradiol (E2) durante grande parte da vida da mulher, que Ă© o tipo mais ativo e estĂĄ relacionado com o ciclo menstrual. Durante a menopausa, ocorre uma diminuição dos nĂveis de E2, responsĂĄvel por vĂĄrias mudanças no organismo. Por exemplo, tem sido relacionado com uma diminuição das infeçÔes vulvovaginais causadas por Candida albicans. Este estudo visou estudar a relação entre a concentração de estrogĂ©nios e alteraçÔes no comportamento destes microrganismos, que poderiam contribuir para a sua patogenicidade. Onze estirpes clĂnicas de C. albicans em estudo foram submetidas a vĂĄrios testes na presença de E1 ou E2: ensaio mĂnimo de concentração inibitĂłria, ensaio de adesĂŁo in-vitro, ensaio de formação de tubo germinativo e infeção das cĂ©lulas HeLa (derivadas de um carcinoma cervical) e cĂ©lulas Hec-1-A (do endomĂ©trio). Tanto a estrona como o estradiol nĂŁo influenciaram o crescimento das estirpes de C. albicans. No entanto, E1 provocou uma maior adesĂŁo in-vitro, e uma maior concentração estrogĂ©nica conduziu a um aumento da formação de tubo germinativo. AlĂ©m disso, houve resposta Ă estimulação hormonal no contexto da infeção, particularmente com as cĂ©lulas Hec-1-A. Em conclusĂŁo, observou-se uma interação entre os estrogĂ©nios e C. albicans, relacionada com fatores de virulĂȘncia, em que a E1 teve mais impacto do que a E2.
Estrogens are sex steroid hormones that, in women, are responsible for the development of the reproductive system. There are several types of estrogens with different functions. Estrone (E1) is considered a weak estrogen and exists at a lower concentration than estradiol (E2), which is the most active type and is related to the menstrual cycle. During menopause, a decrease of E2 levels occurs, which has several impacts. For example, it has been related with a decrease in vulvovaginal infections caused by Candida albicans. This study aimed to investigate the relationship between the concentration of estrogens and alterations in C. albicans behaviour, that could contribute to its pathogenesis. Eleven clinical C. albicans strains under study were subjected to several tests in the presence of E1 or E2: minimum inhibitory concentration test, in-vitro adhesion test, germinative tube formation test, and infection of HeLa cells (derived from a cervical carcinoma) and Hec-1-A cells (from the endometrium). Both E1 and E2 did not impair the growth of C. albicans strains. However, E1 prompted a greater in-vitro adhesion, and an increased estrogenic concentration lead to an increase in germ tube formation. In addition, there was a high response to hormones stimulation in the context of infection, particularly with Hec-1-A cells. In conclusion, it was observed, an interaction between estrogens and C. albicans strains, related to the phenotypic expression of virulence factors and not their viability in which E1 had more impact than E2.
Estrogens are sex steroid hormones that, in women, are responsible for the development of the reproductive system. There are several types of estrogens with different functions. Estrone (E1) is considered a weak estrogen and exists at a lower concentration than estradiol (E2), which is the most active type and is related to the menstrual cycle. During menopause, a decrease of E2 levels occurs, which has several impacts. For example, it has been related with a decrease in vulvovaginal infections caused by Candida albicans. This study aimed to investigate the relationship between the concentration of estrogens and alterations in C. albicans behaviour, that could contribute to its pathogenesis. Eleven clinical C. albicans strains under study were subjected to several tests in the presence of E1 or E2: minimum inhibitory concentration test, in-vitro adhesion test, germinative tube formation test, and infection of HeLa cells (derived from a cervical carcinoma) and Hec-1-A cells (from the endometrium). Both E1 and E2 did not impair the growth of C. albicans strains. However, E1 prompted a greater in-vitro adhesion, and an increased estrogenic concentration lead to an increase in germ tube formation. In addition, there was a high response to hormones stimulation in the context of infection, particularly with Hec-1-A cells. In conclusion, it was observed, an interaction between estrogens and C. albicans strains, related to the phenotypic expression of virulence factors and not their viability in which E1 had more impact than E2.
Description
Keywords
Candida Albicans Candidose Vulvovaginal Estradiol Estrona Fatores de VirulĂȘncia