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Advisor(s)
Abstract(s)
Introdução: Os disruptores são compostos naturais ou sintéticos que podem perturbar a
homeostasia do sistema endócrino em humanos e animais. Há alguns medicamentos
definidos como possíveis disruptores endócrinos. O diclofenac (DCF) é um antiinflamatório não esteroide (AINE) enquanto que o paracetamol (PAR) é um fármaco
analgésico e antipirético, sendo dos medicamentos mais utilizados a nível mundial.
Recentemente, alguns estudos sugeriram que o paracetamol e o diclofenac podem ter um
potencial disruptor endócrino interferindo na homeostasia hormonal sexual. No entanto,
seu efeito vascular enquanto disruptor endócrino nunca foi estudado.
Objetivo: Assim, o objetivo deste estudo é investigar os efeitos do paracetamol e do
diclofenac nas células do músculo liso da aorta do rato, sem endotélio, a fim de analisar seus
potenciais efeitos não genómicos independentes do endotélio.
Material e Métodos: Utilizando a técnica padrão de banho de órgãos, contraíram-se os
anéis aórticos de ratos sem endotélio com noradrenalina (NA) a 1µM e cloreto de potássio
(KCl) a 60mM. Os efeitos do paracetamol e do diclofenac foram analisados em diferentes
concentrações crescentes.
Resultados: Os resultados preliminares demonstraram que o paracetamol induz um
relaxamento da aorta, sendo este efeito vasorelaxante maior nas artérias previamente
contraídas com NA do que com KCl. No que concerne ao diclofenac, os resultados
demonstram uma menor atividade vasorrelaxante que o paracetamol. O efeito
vasorelaxante significativo máximo foi observado nas artérias contraídas com KCl. Por
outro lado, os resultados sugerem que o diclofenac não apresenta um efeito relaxante
significativo nas aortas de ratos contraídas com NA.
Discussão e Conclusão: Os dois fármacos induzem a um relaxamento nas células do
músculo liso vascular de rato sendo necessários mais estudos para esclarecer o seu modo de
ação vascular. Estes efeitos parecem ser similares aos do estrogénio a nível vascular o que
poderá corroborar os trabalhos de investigação que apontam estes fármacos como tendo
uma ação disruptora endócrina estrogénica.
Introduction: Endocrine disruptors are natural or synthetic compounds that can disturb the homeostasis of the endocrine system in humans and animals. There are a few drugs that have been defined as possible endocrine disruptors. Diclofenac (DCF)is a nonsteroidal antiinflammatory drug (NSAID) and Paracetamol (PAR) is an analgesic and antipyretic drug, both are among the most used drugs worldwide. Recently, some studies suggested that paracetamol and diclofenac can have endocrine disruptive potential through interference with sexual hormonal homeostasis. However, its vascular effect as an endocrine disruptor has never been analysed. Objective: In this sense, the aim of this study is to analyse the Paracetamol and Diclofenac effects on the rat aortic smooth muscle cells without endothelium in order to analyse its potential non-genomic effects independent of endothelium. Material and Methods: Using a standard organ bath technique, rat aortic rings without endothelium were contracted with noradrenaline (NA) at 1µM and Potassium chloride (KCl) at 60mM. The effects of Paracetamol and Diclofenac were analysed at different concentrations. Results: Preliminary results indicated that Paracetamol induces aorta relaxation with a higher vasorelaxant effect in the arteries previously contracted with NA than with KCl. Regarding Diclofenac, ours results demonstrate a lower vasorelaxant activity than paracetamol. The maximal significant vasorelaxant effect was observed in the arteries contracted with KCl. On the other hand, the results suggest that Diclofenac has no significant relaxant effect in rat aortas contracted with NA. Discussion and Conclusion: The two drugs induce relaxation on rat vascular smooth muscle cells and further studies are needed to clarify their vascular mode of action. These effects appear to be similar to those of estrogen on vascular level, what can confirm the researches that suggest that these drugs can have a estrogenic endocrine disruptive action.
Introduction: Endocrine disruptors are natural or synthetic compounds that can disturb the homeostasis of the endocrine system in humans and animals. There are a few drugs that have been defined as possible endocrine disruptors. Diclofenac (DCF)is a nonsteroidal antiinflammatory drug (NSAID) and Paracetamol (PAR) is an analgesic and antipyretic drug, both are among the most used drugs worldwide. Recently, some studies suggested that paracetamol and diclofenac can have endocrine disruptive potential through interference with sexual hormonal homeostasis. However, its vascular effect as an endocrine disruptor has never been analysed. Objective: In this sense, the aim of this study is to analyse the Paracetamol and Diclofenac effects on the rat aortic smooth muscle cells without endothelium in order to analyse its potential non-genomic effects independent of endothelium. Material and Methods: Using a standard organ bath technique, rat aortic rings without endothelium were contracted with noradrenaline (NA) at 1µM and Potassium chloride (KCl) at 60mM. The effects of Paracetamol and Diclofenac were analysed at different concentrations. Results: Preliminary results indicated that Paracetamol induces aorta relaxation with a higher vasorelaxant effect in the arteries previously contracted with NA than with KCl. Regarding Diclofenac, ours results demonstrate a lower vasorelaxant activity than paracetamol. The maximal significant vasorelaxant effect was observed in the arteries contracted with KCl. On the other hand, the results suggest that Diclofenac has no significant relaxant effect in rat aortas contracted with NA. Discussion and Conclusion: The two drugs induce relaxation on rat vascular smooth muscle cells and further studies are needed to clarify their vascular mode of action. These effects appear to be similar to those of estrogen on vascular level, what can confirm the researches that suggest that these drugs can have a estrogenic endocrine disruptive action.
Description
Keywords
Células Musculares Lisas da Aorta de Rato Diclofenac Disruptor Endócrino Estrogénio Paracetamol