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Authors
Abstract(s)
O presente trabalho encontra-se dividido em dois capítulos que descrevem a minha
experiência profissionalizante para obtenção do grau de Mestre em Ciências Farmacêuticas,
apresentando uma componente de investigação, bem como uma componente de estágio
realizado em Farmácia Comunitária.
O primeiro capítulo é respeitante à componente de investigação laboratorial desenvolvida
no Centro de Investigação em Ciências da Saúde da Universidade da Beira Interior e
intitula-se “Bioatividade de extratos de Echinacea purpurea, um potencial ingrediente
cosmético”.
A procura por ingredientes naturais na indústria cosmética tem aumentado devido às suas
propriedades antimicrobianas na preservação de formulações, bem como ao seu potencial
antioxidante, que pode ser interessante em produtos antienvelhecimento. A Echinacea
purpurea é cultivada nos EUA e na Europa, não apenas pela sua beleza natural, mas
também pelas suas propriedades médicas reportadas, que incluem estimulação do sistema
imunológico e atividade antimicrobiana.
Posto isto, o objetivo foi averiguar a potencialidade de dois extratos de Echinacea purpurea,
Infusão Aquosa (IA) e extrato Hidroalcoólico (HA), para serem utilizados como
ingredientes ativos na formulação de produtos cosméticos. Para tal, a metodologia aplicada
centrou-se na caracterização da bioatividade dos extratos. A atividade antimicrobiana foi
determinada pelo ensaio de microdiluição contra microrganismos incluídos no Challenge
Test de cosméticos (S. aureus, P. aeruginosa, E. coli, C. albicans, A. brasiliensis), bem como
microrganismos que fazem parte da microbiota da pele (S. epidermidis, C. amycolatum).
As atividades antioxidante e citotóxica foram determinadas pelo ensaio de redução DPPH e
pelo ensaio MTT numa linha celular de fibroblastos de pele de ratinho (NIH / 3T3; ATCC
CRL-1658), respetivamente.
Os resultados obtidos revelaram que os extratos de Echinacea purpurea, principalmente o
extrato HA, por apresentar atividade antifúngica, modesta atividade antioxidante e
biocompatibilidade celular, em concentrações específicas, pode ter potencial como
ingrediente conservante (adjuvante) e antioxidante em produtos cosméticos. No entanto,
este estudo constituiu uma avaliação primária ao potencial destes extratos enquanto
ingredientes cosméticos, pelo que, futuramente, devem desenvolver-se estudos que incluam
outras avaliações tanto de eficácia como de segurança.
No que diz respeito ao segundo capítulo, este é alusivo ao estágio em farmácia comunitária,
realizado na Farmácia Social Mutualista Covilhanense, na cidade da Covilhã, que se revelou
crucial para a minha formação enquanto futura farmacêutica. Este capítulo tem como
objetivo descrever a experiência e aprendizagem que me foram transmitidas relativamente
ao funcionamento e prática do dia-a-dia de uma farmácia comunitária.
The present work is divided in two chapters that describe my professional experience to obtain the Master's degree in Pharmaceutical Sciences, presenting a research component, as well as an internship component carried out in Community Pharmacy. The first chapter concerns the laboratory research component developed at the Health Sciences Research Center of the University of Beira Interior and is entitled “Bioactivity of Echinacea purpurea extracts, a potential cosmetic ingredient”. The search for natural ingredients in cosmetic industry has increased due to their antimicrobial properties in the preservation of formulations, as well as their antioxidant potential, which may be interesting in anti-aging products. Echinacea purpureais cultivated throughout the USA and Europe not only because of its natural beauty but also for its reported medical properties, which includes stimulation of immunologic system and antimicrobial activity. Therefore, the goal was to investigate the potential of two extracts of Echinacea purpurea, Aqueous Infusion (AI) and Hydroalcoholic Extract (HA), as active ingredients in the formulation of cosmetic products. To do this, the applied methodology focused on the characterization of the extracts' bioactivity. The antimicrobial activity was determined by the microdilution test against microorganisms included in the Cosmetics Challenge Test (S. aureus, P. aeruginosa, E. coli, C. albicans, A. brasiliensis), as well as microorganisms that are part of the skin microbiota (S. epidermidis, C. amycolatum). Antioxidant and cytotoxic activities were determined by the DPPH reduction assay and the MTT assay on a rat skin fibroblast cell line (NIH/3T3; ATCC CRL-1658), respectively. The results obtained revealed that Echinacea purpureaextracts, mainly the HA extract, for presenting antifungal activity, modest antioxidant activity and cellular biocompatibility, at specific concentrations, may have potential to be used as a preservative and antioxidant ingredient in cosmetic products. However, this study was a primary assessment of the potential of these extracts as cosmetic ingredients, so, in the future, studies should be carried out toinclude other assessments of both efficacy and safety. With regard to the second chapter, this alludes to the internship in community pharmacy, held at the Farmácia Social Mutualista Covilhanense, in the city of Covilhã, which proved to be crucial for my training as a future pharmacist. The purpose of this chapter is to describe the experience and learning that I have been given regarding the day-to-day functioning and practice of a community pharmacy.
The present work is divided in two chapters that describe my professional experience to obtain the Master's degree in Pharmaceutical Sciences, presenting a research component, as well as an internship component carried out in Community Pharmacy. The first chapter concerns the laboratory research component developed at the Health Sciences Research Center of the University of Beira Interior and is entitled “Bioactivity of Echinacea purpurea extracts, a potential cosmetic ingredient”. The search for natural ingredients in cosmetic industry has increased due to their antimicrobial properties in the preservation of formulations, as well as their antioxidant potential, which may be interesting in anti-aging products. Echinacea purpureais cultivated throughout the USA and Europe not only because of its natural beauty but also for its reported medical properties, which includes stimulation of immunologic system and antimicrobial activity. Therefore, the goal was to investigate the potential of two extracts of Echinacea purpurea, Aqueous Infusion (AI) and Hydroalcoholic Extract (HA), as active ingredients in the formulation of cosmetic products. To do this, the applied methodology focused on the characterization of the extracts' bioactivity. The antimicrobial activity was determined by the microdilution test against microorganisms included in the Cosmetics Challenge Test (S. aureus, P. aeruginosa, E. coli, C. albicans, A. brasiliensis), as well as microorganisms that are part of the skin microbiota (S. epidermidis, C. amycolatum). Antioxidant and cytotoxic activities were determined by the DPPH reduction assay and the MTT assay on a rat skin fibroblast cell line (NIH/3T3; ATCC CRL-1658), respectively. The results obtained revealed that Echinacea purpureaextracts, mainly the HA extract, for presenting antifungal activity, modest antioxidant activity and cellular biocompatibility, at specific concentrations, may have potential to be used as a preservative and antioxidant ingredient in cosmetic products. However, this study was a primary assessment of the potential of these extracts as cosmetic ingredients, so, in the future, studies should be carried out toinclude other assessments of both efficacy and safety. With regard to the second chapter, this alludes to the internship in community pharmacy, held at the Farmácia Social Mutualista Covilhanense, in the city of Covilhã, which proved to be crucial for my training as a future pharmacist. The purpose of this chapter is to describe the experience and learning that I have been given regarding the day-to-day functioning and practice of a community pharmacy.
Description
Keywords
Atividade Antimicrobiana Atividade Antioxidante Atividade Citotóxica Echinacea
Purpurea Farmácia Comunitária Ingredientes Produtos Cosméticos