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Abstract(s)
Ao longo das últimas décadas tem-se registado um aumento do consumo de suplementos alimentares à base de plantas. Muitos destes produtos têm demonstrado interagir com medicamentos, alguns deles com margem terapêutica estreita. Este trabalho experimental pretendeu estudar o potencial de interacção entre o extracto de Fucus vesiculosus e a amiodarona, ambos administrados pela via oral, no rato.
Para avaliar o potencial de interacção foram desenhados 2 estudos experimentais: um de administração única e concomitante de extracto de Fucus vesiculosus e amiodarona, e o segundo que envolveu a administração do extracto aos ratos durante 14 dias e a administração de amiodarona no 15º dia. A amostra compreendeu 20 ratos Wistar. Foram recolhidas amostras de plasma e de órgãos (coração, fígado e pulmão), as quais após processamento mediante extracção liquido-liquido (LLE) foram analisadas através de HPLC-DAD.
Os resultados indicam que o extracto de Fucus vesiculosus administrado por via oral, em simultâneo e em dose única com amiodarona, influencia a velocidade e extensão de exposição sistémica ao fármaco, possivelmente por interferência na absorção ao nível do tracto gastrointestinal. Tanto a Cmax, como a AUCo-t e a AUC0-∞ sofreram uma diminuição considerável quando a amiodarona foi administrada com o extracto de Fucus vesiculosus, em comparação com o respectivo grupo controlo (amiodarona + veículo do extracto). Por outro lado, não foram detectadas diferenças importantes na cinética plasmática da amiodarona no estudo em que se administrou o extracto de Fucus vesiculosus em dose múltipla durante 14 dias e a amiodarona ao 15º dia. A concentração de amiodarona, ou do seu metabolito mono-N-desetilamiodarona (MDEA), nos órgãos, não parece ser significativamente afectada pela administração do extracto de Fucus vesiculosus, mesmo após a administração concomitante de Fucus vesiculosus e amiodarona, apesar das alterações registadas nos níveis plasmáticos.
Em conclusão, estes dados demonstram algum potencial de interacção entre o extracto de Fucus vesiculosus e a amiodarona, um fármaco de margem terapêutica estreita, quando administrados concomitantemente ao rato. Mas, apesar de tal ocorrer, parece não haver uma correlação entre esta interacção e as concentrações de amiodarona e MDEA nos órgãos, nomeadamente no coração, o órgão-alvo deste fármaco.
Over the past decades there has been an increased consumption of herbal dietary supplements. Many of these products have been shown to interact with medications, some of them with a narrow therapeutic index. This experimental work sought to study a potential interaction between a Fucus vesiculosus extract and amiodarone, both administered orally to rats. To ascertain the potential of interactions, 2 experimental studies were designed: a single administration study with concomitant administration of a Fucus vesiculosus extract and amiodarone, and the second envolving the administration of the extract to rats for 14 days, and amiodarone on the 15th day. The sample included 20 Wistar rats. Plasma and organ samples (heart, liver and lung) were collected, processed by liquid-liquid extraction (LLE), and analysed by HPLC-DAD. The Fucus vesiculosus extract administered orally, simultaneously and in a single-dose with amiodarone, influences the rate and extension of systemic drug exposure, possibly derived from interference in the gastrointestinal tract absorption. Both Cmax, AUC0-t and AUC0-∞ suffered a considerable decrease when amiodarone was administered with the extract of Fucus vesiculosus, compared with the respective control group (amiodarone + vehicle of the extract). Oh the other hand, no significant differences in the amiodarone plasma kinetics were detected, in the multiple dose study, wich comprised the administration of the Fucus vesiculosus extract for 14 days and the administration of amiodarone on the 15th day of the study. The concentrations of amiodarone, or its metabolite mono-N-desethylamiodarone (MDEA), in the organs, did not appear to be significantly affected by the extract of Fucus vesiculosus, even after the administration, at the same time, of the Fucus vesiculosus extract and amiodarone, despite the changes recorded in the plasma levels. In conclusion, the results show some potential for an interaction between the Fucus vesiculosus extract and amiodarone, a drug with a narrow therapeutic index, when these are administered to rats, at the same time. But, despite this occurs, it seems there isn´t a correlation between the interaction and the concentrations of amiodarone and MDEA in the organs. The amiodarone and MDEA concentrations in the heart, the target-organ for these molecules, are not affected by the Fucus vesiculosus extract.
Over the past decades there has been an increased consumption of herbal dietary supplements. Many of these products have been shown to interact with medications, some of them with a narrow therapeutic index. This experimental work sought to study a potential interaction between a Fucus vesiculosus extract and amiodarone, both administered orally to rats. To ascertain the potential of interactions, 2 experimental studies were designed: a single administration study with concomitant administration of a Fucus vesiculosus extract and amiodarone, and the second envolving the administration of the extract to rats for 14 days, and amiodarone on the 15th day. The sample included 20 Wistar rats. Plasma and organ samples (heart, liver and lung) were collected, processed by liquid-liquid extraction (LLE), and analysed by HPLC-DAD. The Fucus vesiculosus extract administered orally, simultaneously and in a single-dose with amiodarone, influences the rate and extension of systemic drug exposure, possibly derived from interference in the gastrointestinal tract absorption. Both Cmax, AUC0-t and AUC0-∞ suffered a considerable decrease when amiodarone was administered with the extract of Fucus vesiculosus, compared with the respective control group (amiodarone + vehicle of the extract). Oh the other hand, no significant differences in the amiodarone plasma kinetics were detected, in the multiple dose study, wich comprised the administration of the Fucus vesiculosus extract for 14 days and the administration of amiodarone on the 15th day of the study. The concentrations of amiodarone, or its metabolite mono-N-desethylamiodarone (MDEA), in the organs, did not appear to be significantly affected by the extract of Fucus vesiculosus, even after the administration, at the same time, of the Fucus vesiculosus extract and amiodarone, despite the changes recorded in the plasma levels. In conclusion, the results show some potential for an interaction between the Fucus vesiculosus extract and amiodarone, a drug with a narrow therapeutic index, when these are administered to rats, at the same time. But, despite this occurs, it seems there isn´t a correlation between the interaction and the concentrations of amiodarone and MDEA in the organs. The amiodarone and MDEA concentrations in the heart, the target-organ for these molecules, are not affected by the Fucus vesiculosus extract.
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Keywords
Focus vesiculosus - Amiodarona - Interacção - Estudo Plantas medicinais - Focus vesiculosus
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Universidade da Beira Interior