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Abstract(s)
A cor é uma das principais características dos objetos que nos rodeiam e enquanto consumidores é um fator determinante na seleção de qualquer produto. A tendência mundial para uso de produtos mais “eco-friendly” e naturais está em grande expansão, e nesse mercado os pigmentos microbianos surgem como uma alternativa na substituição de corantes sintéticos em diferentes indústrias.
A prodigiosina produzida por Serratia plymuthica, bactéria gram negativa da família das Enterobacteriaceae, é um metabólito secundário de produção intracelular. Para além do potencial de tingimento, possui atividades biológicas (antifúngica, imunossupressora, anticancerígena e antimicrobiana) que podem ser exploradas, proporcionando a obtenção de materiais multifuncionais. O presente estudo teve por objetivo produzir o pigmento prodigiosina, alcançar as melhores condições de crescimento da bactéria e produção do pigmento, definir a concentração mínima inibitória (MIC) do pigmento contra Staphylococcus aureus (SA) e Pseudomonas aeruginosa (PA), funcionalizar uma amostra têxtil de algodão e avaliar o efeito antibacteriano da prodigiosina após processo de funcionalização no algodão.
As melhores condições de cultivo alcançadas foram em incubações a 22ºC ± 2ºC por 48h em meio de cultura PGP (glicerol como fonte de carbono e peptona como fonte de nitrogénio), taxa de oxigenação correspondente a 20% (v/v) e agitação (nos cultivos submersos) entre 150 e 200rpm. Obteve-se valores de MIC de 0,24 ?g/mL e 1,25 mg/mL para, respectivamente, SA e PA. E a avaliação da eficácia da funcionalização demonstrou que o pigmento ficou ligado a fibra e possui atividade antibacteriana, pois reduziu o crescimento bacteriano em 97,10% (SA) e 45,26% (PA), em comparação com resultados da amostra sem prodigiosina.
The color of any object is, for any consumer, a determining factor in products selection. The worldwide trend towards more "eco-friendly" and natural products is expanding, and in this market microbial pigments emerge as an alternative to replace synthetic dyes in several industries. The prodigiosin produced by Serratia plymuthica, a gram-negative bacterium of the family Enterobacteriaceae, is a secondary metabolite of intracellular production. Besides the coloring properties, the pigment has many biological activities (antifungal, immunosuppressive, non-carcinogenic and antimicrobial) that can be further exploited to obtain materials with multifunctional properties. The aim of the present study was to produce the pigment prodigiosin, to achieve the best growth conditions for the bacteria and pigment production, to define the minimum inhibitory concentration (MIC) of the pigment against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA), to functionalize a sample of cotton textile with pigment and to evaluate the antibacterial effect of prodigiosin after the functionalization process. The best culture conditions were achieved in incubations at 22 ºC ± 2ºC for 48h in a PGP culture (glycerol as carbon source and peptone as nitrogen source), oxygenation rate of 20% (v / v) and agitation (in submerged cultures) between 150 and 200rpm. MIC values of 0.24 mg / mL and 1.25 mg / mL were obtained for, respectively, SA and PA. And the evaluation of the effectiveness of the functionalization showed that the pigment was bound to fiber and has antibacterial activity, as it reduced bacterial growth by 97.10% (SA) and 45.26% (PA), compared to results of the sample without prodigiosin.
The color of any object is, for any consumer, a determining factor in products selection. The worldwide trend towards more "eco-friendly" and natural products is expanding, and in this market microbial pigments emerge as an alternative to replace synthetic dyes in several industries. The prodigiosin produced by Serratia plymuthica, a gram-negative bacterium of the family Enterobacteriaceae, is a secondary metabolite of intracellular production. Besides the coloring properties, the pigment has many biological activities (antifungal, immunosuppressive, non-carcinogenic and antimicrobial) that can be further exploited to obtain materials with multifunctional properties. The aim of the present study was to produce the pigment prodigiosin, to achieve the best growth conditions for the bacteria and pigment production, to define the minimum inhibitory concentration (MIC) of the pigment against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA), to functionalize a sample of cotton textile with pigment and to evaluate the antibacterial effect of prodigiosin after the functionalization process. The best culture conditions were achieved in incubations at 22 ºC ± 2ºC for 48h in a PGP culture (glycerol as carbon source and peptone as nitrogen source), oxygenation rate of 20% (v / v) and agitation (in submerged cultures) between 150 and 200rpm. MIC values of 0.24 mg / mL and 1.25 mg / mL were obtained for, respectively, SA and PA. And the evaluation of the effectiveness of the functionalization showed that the pigment was bound to fiber and has antibacterial activity, as it reduced bacterial growth by 97.10% (SA) and 45.26% (PA), compared to results of the sample without prodigiosin.
Description
Keywords
Antibacteriano Condições de Crescimento Pigmentos Microbianos Prodigiosina Serratia Plymuthica