Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.6/4690
Título: 3D tumor spheroids: an overview on the tools and techniques used for their analysis
Autor: Costa, Elisabete Cristina da Rocha
Moreira, André Ferreira
Diogo, Duarte Miguel de Melo
Gaspar, Vítor Manuel Abreu
Carvalho, Marco António Paulo de
Correia, Ilídio Joaquim Sobreira
Palavras-chave: 3D tumor spheroids
Cancer therapy
In vitro models
Therapeutic screening
Data: 11-Nov-2016
Editora: Elsevier
Resumo: In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids.
Peer review: yes
URI: http://hdl.handle.net/10400.6/4690
DOI: 10.1016/j.biotechadv.2016.11.002
Versão do Editor: https://www.sciencedirect.com/science/article/pii/S0734975016301379
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