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- The role of STEAP1 in the sensitivity of C4-2B prostate cancer cells to taxane-based chemotherapyPublication . Coelho, Rafaella Severino; Baptista, Cláudio Jorge Maia; Rocha, Sandra Catarina Moreira daProstate cancer (PCa) is the third-leading cause of cancer death in men worldwide. As a precursor to PCa, prostatic intraepithelial neoplasia (PIN) lesions are defined as a preneoplastic growth of the prostate that could develop into carcinoma and metastatic disease. The Six Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) is a prostate-specific cell-surface antigen, almost exclusively expressed on the secretory epithelium of the prostate tissue. However, this protein is known to be overexpressed in several types of cancer, including PCa. The STEAP1 protein is found on cell-cell junctions and acts as an ion channel or transporter of small molecules, indicating that this protein plays an important role in cell communication. In cancer, STEAP1 overexpression has been associated with inhibition of apoptosis, enhancement of cell proliferation, migration, and invasion, as well as induction of epithelial to mesenchymal transition. Furthermore, a link between overexpression of STEAP1 and poor prognosis in PCa has also been found. The most common approach for metastatic castration-resistant PCa treatment is the sequential prescription of single-agent therapies. Among those agents are the so-called taxane-based chemotherapeutics, a category of highly useful antineoplastic drugs, which includes paclitaxel, docetaxel, and cabazitaxel. However, as the disease progresses, patients become resistant to treatment. Currently, up to 90 % of cancerrelated deaths are due to the emergence of drug resistance. Nonetheless, combined therapies have been explored in an attempt to overcome the resistance and improve the therapeutic effectiveness. This study aimed to assess the sensitivity of PCa cells to taxane-based drugs when the STEAP1 gene is silenced, as well as to evaluate the expression of STEAP1 in response to the same drugs. C4-2B cells were selected to be transfected with a specific siRNA against the STEAP1 gene or with a scramble siRNA sequence. Wild-type and STEAP1-knockdown C4-2B cells were treated with paclitaxel, docetaxel, or cabazitaxel, and STEAP1 expression and cell viability were evaluated. The results showed that STEAP1 knockdown or taxane-based treatment significantly reduced the viability of PCa cells. However, the effect was reversed when the drugs were combined with STEAP1 knockdown, where the cell viability of C4-2B cells increased. This study demonstrated that STEAP1 expression levels might influence the response of PCa cells to chemotherapeutics drugs. Moreover, it seems that the use of paclitaxel, docetaxel, or cabazitaxel is more effective in PCa cells that overexpress STEAP1.
- Relatório de Estágio Académico de Viseu Futebol Clube, SUB-19Publication . Costa, Pedro Miguel Gomes; Ferraz, Ricardo Manuel Pires; Branquinho, Luís Filipe CardosoNo âmbito da Unidade Curricular de Estágio do 2º ciclo de estudos em Ciências do Desporto – Treino Desportivo, da Universidade da Beira Interior (UBI), foi realizado um estágio curricular no Académico de Viseu Futebol Clube (AVFC), equipa que competiu no Campeonato Nacional Sub-19 2ª Divisão. Atividades realizadas no decorrer deste estágio, decorreram na equipa técnica do plantel de Sub-19 na categoria de treinador estagiário. O treino desportivo tem evoluído significativamente nas últimas décadas, especialmente no futebol, onde várias metodologias têm sido desenvolvidas de forma a otimizar o desempenho dos jogadores. Assim com o intuito de potenciar o desenvolvimento do processo de treino, no decorrer do estágio foi realizado um trabalho de investigação que teve como principal objetivo de investigar os efeitos de diferentes tempos de recuperação nas respostas de carga de treino durante a realização de jogos reduzidos de 4 vs. 4. Participaram no estudo, 8 jogadores da equipa de sub-19 que participavam no campeonato nacional da categoria. Foram utilizados dois formatos de jogo reduzido com a duração total de 18 minutos: 1 contínuo (18 minutos) e 4 fracionados (3x6 minutos) com diferentes tempos de recuperação entre repetições (30s, 1 min, 1,30m e 2min). Os resultados permitiram concluir que existe uma tendência para respostas mais elevadas de carga de treino com o uso de métodos fracionados e períodos mais longos de recuperação com exceção da velocidade máxima onde curtos períodos de recuperação (30s) parecem ser suficientes para promover altas respostas de carga externa. A comparação entre métodos fracionado e contínuo sugere que o método fracionado resulta em cargas interna e externa mais elevadas.