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  • Angiotensin-converting enzyme genotype affects skeletal muscle strength in elite athletes
    Publication . Costa, Aldo M.; Silva, António; Garrido, Nuno; Louro, Hugo; Marinho, Daniel; Marques, MC; Granadeiro, Luiza Breitenfeld
    Previous studies have associated angiotensin-converting enzyme (ACE) D allele with variability in the skeletal muscle baseline strength, though conclusions have been inconsistent across investigations. The purpose of this study was to examine the possible association between ACE genotype and skeletal muscle baseline strength in elite male and female athletes involved in different event expertise. A group of 58 elite athletes, designated as Olympic candidates, were studied: 35 swimmers (19 males and 16 females, 18.8 ± 3.2 years) and 23 triathletes (15 males and 8 females, 18.7 ± 3.0 years). The athletes were classified as: short (≤ 200m) and middle (400m to 1500m) distance athletes, respectively. For each subject the grip strength in both hands was measure using an adjustable mechanical hand dynamometer. The maximum height in both squat jump (SJ) and counter movement jump (CMJ) were also assessed, using a trigonometric carpet (Ergojump Digitime 1000; Digitest, Jyvaskyla, Finland). DNA extraction was obtained with Chelex 100(®) and genotype determination by PCR-RFLP methods. Both males and females showed significantly higher right grip strength in D allele carriers compared to II homozygote's. We found that allelic frequency differs significantly by event distance specialization in both genders (p < 0.05). In fact, sprinter D allele carriers showed the superior scores in nearly all strength measurements (p < 0.05), in both genders. Among endurance athletes, the results also demonstrated that female D allele carriers exhibited the higher performance right grip and CMJ scores (p < 0.05). In conclusion, the ACE D allele seems associated with skeletal muscle baseline strength in elite athletes, being easily identified in females. Key pointsDD homozygote's and D allele carriers from both genders shows significantly higher right grip strength.Right grip strength remains significantly higher in the D allele carrier's female endurance group.Female's D allele carriers exhibited the higher performance counter-movement jump scores.ACE genotype effects in skeletal-muscle strength are diverse by gender, being easily identified in females.
  • Tracking the performance, energetics and biomechanics of international versus national level swimmers during a competitive season
    Publication . Costa, Mário Jorge; Bragada, José; Mejias, Jean Erik Gonçalves; Louro, Hugo; Marinho, Daniel; Silva, António; Barbosa, Tiago M.
    The purpose of this study was to track and compare the changes of performance, energetic and biomechanical profiles of international (Int) and national (Nat) level swimmers during a season. Ten Portuguese male swimmers (four Int and six Nat level subjects) were evaluated on three different time periods (TP(1), TP(2), TP(3)) of the 2009-2010 season. Swimming performance was assessed based on official time's lists of the 200-m freestyle event. An incremental set of 7 × 200 m swims was applied to assess the energetic and biomechanical data. Measurements were made of: (1) velocity at the 4 mmol of lactate levels (V4), stroke index at V4 (SI@V4) and propelling efficiency at V4 (η (p)@V4), as energetic estimators; (2) stroke length at V4 (SL@V4) and stroke frequency at V4 (SF@V4), as biomechanical variables. The results demonstrated no significant variations in all variables throughout the season. The inter-group comparison pointed out higher values for Int swimmers, with statistical differences for the 200 m performance in all time periods. Near values of the statistical significance were demonstrated for the SI@V4 in TP(1) and TP(3). The tracking based on K values was high only for the SI@V4. It is concluded that a high stability can be observed for elite swimmers performance, energetic and biomechanical profiles throughout a single season. Int swimmers are able to maintain a higher energetic and biomechanical capacity than Nat ones at all times. The SI@V4 may be used as an indicator of performance variation.
  • Effects of swim training on energetics and performance
    Publication . Costa, Mário Jorge; Bragada, José; Mejias, Jean Erik Gonçalves; Louro, Hugo; Marinho, Daniel; Silva, António; Barbosa, Tiago M.
    The aim of this study was to determine the effect of several months of training on performance and energetic profile of elite swimmers. 9 elite swimmers were evaluated at 3 different time periods during the 2010-2011 calendar. Swimming performance was assessed based on lists of times for the 200 m freestyle event. An incremental set of 7×200 m swims was applied to obtain the energetic data. Measurements and/or estimations were made for the: velocity at 4 mmol l(-1) of lactate concentrations, highest value of lactate concentrations, maximal oxygen consumption, minimum swimming velocity where the maximal oxygen consumption is reached and total energy expenditure (Etot). The performance and most of the energetic variables assessed presented no significant variations during the study period. The only exception was the Etot with significant differences between all measurements. Correlation coefficients suggested a high stability for all variables. Cohen's Kappa tracking index demonstrated high variability in the individual adaptations to training. It is concluded that elite swimmers demonstrate a slight improvement in performance and energetic profile in response to several months of training. Each subject has an individual way of adapting to the training load, combining the different energetic confounders to enhance performance.