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- Material de Construção do Ninho da Vespa Asiática - Um contributoPublication . Sedira, Naim; Gomes, Ana; Nepomuceno, Miguel; Paiva, Anabela; Bentes, I.; Pinto, Jorge Tiago Queirós da Silva; Pereira, SandraMuitos trabalhos de investigação têm vindo a ser desenvolvidos no sentido de encontrar novas soluções para os diversos contextos da ciência através da bio-inspiração. Este trabalho está centrado no estudo do ninho da vespa asiática e, em particular, no estudo do seu material de construção. Esta construção natural é complexa em todas a vertentes. A sua complexidade arquitetónica, estrutural, material, funcional, entre outros aspetos técnicos, são fatores que despertam a curiosidade, esta construção pode ainda apresentar dimensões relativamente grandes. Ela é construída e usada aproximadamente meio ano e não volta a ser reutilizada. Este é outro aspeto técnico que motiva um estudo tendo em conta a escassez de habitação a nível mundial. O material de construção também é interessante e inovador. Neste ponto, tentou-se compreender a constituição deste material através da realização de ensaios MEV-EDS de amostras do material do ninho retiradas em diferentes zonas. Amostras de folhas de árvore foram analisadas para serem usadas como referência. As imagens de MEV mostram que o ninho da vespa asiática é feito de fibras vegetais e pedaços de madeira mastigadas, com sulcos e poros para manipulação, troca de gases e regulação de temperatura e humidade. A análise EDS do material do ninho de vespas asiáticas indica origem vegetal, com elementos como carbono (C) e oxigénio (O) formando 82,31%, e magnésio (Mg), potássio (K), cálcio (Ca) e ferro (Fe) constituindo 9,43%. Alumínio (Al) e silício (Si) representam 7,41%. A saliva das vespas é crucial para a construção. Importa acrescentar que este material é utilizado na construção da fachada do ninho, a qual, apesar de estar diretamente exposta às intempéries, proporciona uma durabilidade adequada. O interior do ninho da vespa asiática também garante o conforto necessário para a reprodução destes insetos. Este trabalho tem como principal objetivo partilhar com a comunidade cientifica alguns dos resultados alcançados por esta equipa de investigação neste contexto.
- Iberian challenges on cultural built heritage academic researchPublication . Simão, Inês Cunha; Martins-Nepomuceno, Ana M.T.; Nepomuceno, Miguel; Salvado, João Pedro Ferreira; Juan García, Natalia; Campelo-Gaínza, Carlos; Lorente, Jesús Pedro; Amoreira, SofiaThis paper explores some of the challenges involved in conducting academic research in the field of built heritage. These include visiting sites, documenting them, surveying them, and talking to the people responsible for their conservation, management and, in the case of 20th century, authorship. We will discuss and argue that academic research into the built heritage is often interdisciplinary in nature and requires different skills and techniques to those used in other areas of research. The focus will be on the similarities between research fields such as art history and architecture. The aim is to highlight the unique aspects of research in the built heritage field and the tools that researchers consider essential to their work, as well as the real challenges that young researchers face. This will be demonstrated through five examples of research carried out in Portugal and Spain, covering different aspects of monastic architecture, art and culture, traditional building techniques, the role of local museums in conserving heritage, and 20th century architecture. Each project will describe how they were undertaken, what their objectives were, and what challenges they encountered, including how these were overcome or presented as opportunities for further research. Through the presentation of ongoing research on Portuguese Cistercian monasteries, art, architecture and monastic culture in Spain, the work of the 20th century Portuguese architect Manuel Taínha, the resilient corbelled domes considered a true example of the dry-stone technique and now classified as intangible heritage, and the involvement of Portuguese and Spanish local mountain museums in their territorial and historical contexts, this paper shows how these five projects have faced multiple and equal setbacks. While significant progress has been made in establishing a comprehensive information network through continued investment in the digital preservation of the built heritage, we conclude that additional investment in the development of multidisciplinary teams and cross-referencing between different research fields and geographical areas still needs to be made.
- Avaliação In Vitro dos Potenciais Efeitos Terapêuticos e Toxicológicos Associados ao Consumo de AyahuascaPublication . Gonçalves, Joana Domingos; Duarte, Ana Paula Coelho; Alba, Maria Eugénia Gallardo; Luís, Ângelo Filipe SantosAyahuasca is a psychoactive beverage that has been consumed for centuries and was originally used by indigenous tribes in the northwest of the Amazon. This beverage consists of a decoction, thick, oily, and brownish in color, which is originally prepared from the leaves of Psychotria viridis Ruiz & Pav. and the scrapings of the stem of Banisteriopsis caapi (Spruce ex Griseb.) C.V.Morton, although over the years several modifications of this decoction have been developed. Currently, some natural or synthetic adulterants that can mimic the effects of the plants originally used are known. This decoction contains the hallucinogenic compound N,N-dimethyltryptamine (DMT) from Psychotria viridis, and β-carboline alkaloids such as harmine, tetrahydroharmine (THH) and harmaline, which come from Banisteriopsis caapi. DMT is the main psychoactive component of ayahuasca, since it acts as a serotonin receptor (5-HT1A/2A/2C) agonist and, when ingested alone, is metabolized by peripheral monoamine oxidase A (MAO-A), making it if harmless. However, together with the β-carboline alkaloids, it can reach the circulation and the central nervous system, by temporarily inhibiting MAO-A. Additionally, THH also inhibits serotonin reuptake enhancing the effects of DMT. This synergy between compounds is known by the indigenous peoples for about 3000 years. Thus, originally, the tribes resorted to this beverage for therapeutic and religious purposes. It was also used by native healers to treat psychological disorders, stimulate visual creativity and creative thinking. More recently, non-indigenous religious entities in various countries, especially in Latin America, also used this beverage for their ceremonies, namely Barquinha, União do Vegetal and Santo Daime. These last two, currently spread to other continents. In recent decades, the popularity of ayahuasca has increased worldwide, being seen as a natural remedy used for millennia to cure various diseases. Thus, currently this beverage, despite continuing to be used in a traditional way, is also consumed recreationally all over the world, as well as used in modern medicine. When consuming ayahuasca, vomiting, diarrhea and nausea often occur. In addition to these physical symptoms, changes in body temperature, pupil size and immunological, endocrine, and cardiovascular changes were observed. Psychological symptoms such as changes in perception (time, space, and the senses) and at the cognitive level have been also described. Additionally, connections with divine and mythical entities are also described, which is the aspect that motivates its consumption in a religious context. Despite all the effects described, over the years several beneficial properties have been pointed out to ayahuasca, even believing in its therapeutic potential in psychological illnesses, such as depression, anxiety, addiction, and psychological disorders. Additionally, beneficial properties in physiological treatments are also pointed out. In recent decades, the consumption of psychoactive substances has been increasing, namely substances of natural origin, such as ayahuasca. This consumption is often incited by cultural motivations or by spiritual and religious beliefs, making it difficult to measure its worldwide use. According to the literature, the ayahuasca consumption of in controlled environments is not dangerous and is not associated with psychotic episodes. However, the expansion of its consumption has raised some concerns. Although the consumption of DMT source plants is not controlled, it has become a controlled substance in some countries, due to the increase in its demand. However, the legislation regulating psychoactive substances is very variable and may even be ambiguous. Considering the potential of ayahuasca, the first objective of this thesis was to develop an analytical methodology for determine and quantify the main compounds present in ayahuasca decoctions, as well as to understand their behavior throughout the digestive process. The second objective was to carry out a phytochemical characterization of ayahuasca decoctions and to evaluate their potential antioxidant, anti-inflammatory, antimicrobial effects and their therapeutic properties in wound healing and cancer treatment. Thus, initially decoctions, in sufficient quantity to carry out the entire study, of four individual plants commonly used in ayahuasca preparations (P. viridis (leaves), Mimosa hostilis Benth. (root), B. caapi (stem) and Peganum harmala L. (seeds)), a decoction of a commercial mixture and four decoctions of mixtures of two of the above plants (P. viridis and B. caapi, P. viridis and Peganum harmala, Mimosa hostilis and B. caapi and M. hostilis and P. harmala) were prepared. The samples were submitted to three microextraction procedures and later, an analytical method was developed and validated for the determination and quantification of the main compounds of these samples. The Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) technique proved to be the most promising and, therefore, was chosen for application in the samples. The analytical method showed to be linear between 0.16 and 10 μg/mL for β-carbolines and between 0.016 and 1 μg/mL for DMT, with determination coefficients (R2) between 0.9968 and 0.9993. The limit of detection (LOD) and lower limit of quantification (LLOQ) were 0.16 μg/mL (0.016 μg/mL for DMT) and extraction efficiencies ranged from 60 to 88%. The bioavailability and bioaccessibility of the same compounds were also evaluated in vitro. The bioaccessibility of the samples was assessed using an in vitro digestion process, and subsequently their bioavailability was evaluated using the colorectal adenocarcinoma cell line (Caco-2). After quantification, it was verified that the compounds (DMT, harmine, harmaline, harmol, harmalol and THH) were released from the matrix during the in vitro digestion process, becoming bioaccessible. Likewise, some of these compounds, after being incubated with the cell monolayer, were absorbed, becoming bioavailable, without presenting a risk to its integrity. The phytochemical profile of the samples was evaluated, and it was demonstrated that, in general, the samples have a high content of phenolic compounds and flavonoids. These results reflect the antioxidant and anti-inflammatory activities also determined. Additionally, the samples showed antimicrobial properties, with emphasis on the effect of B. caapi and P. harmala on the A. baumannii strain, which resulted in the inhibition of both biofilm formation and quorum sensing. Ayahuasca samples also demonstrated their therapeutic potential, particularly in terms of wound healing. Using normal human dermal fibroblasts (NHDF), a scratch assay was performed, and it was found that only one sample showed cytotoxicity and the others promoted the migration of skin fibroblasts, without cutaneous absorption of DMT and β-carboline alkaloids. Likewise, the ayahuasca samples showed a great anticancer potential in Caco-2 cells, having been verified that after incubation, the samples significantly induced apoptosis while cell proliferation decreased significantly. There was also a significant reduction in oxidative stress with some samples, with a significant increase in the activity of antioxidant enzymes. Similarly, in gastric adenocarcinoma (AGS) cells, an increase in the induction of apoptosis and a reduction in oxidative stress were observed after incubation with the ayahuasca samples. In conclusion, the main results of this thesis demonstrated that ayahuasca does indeed have a great therapeutic potential, with emphasis on its antimicrobial, antioxidant, anti-inflammatory, and healing actions, but above all its anticancer activity studied in two different cell lines. Also noteworthy was the development and validation of the analytical method where three miniaturized extraction techniques were compared and where the QuEChERS technique was applied to ayahuasca samples for the first time.
- CH-Resilience Project (Cultural Heritage: Resilience of Built Heritage and Built Environment): Internationalization and Research Network within the “Universitas Montium” (UNITA) AlliancePublication . Martins, Ana M. T.; Nepomuceno, Miguel C. S.; Simão, Inês Cunha; Juan García, Natalia; Campelo-Gaínza, Carlos; Pedro Lorente, Jesús; Salvado, João Pedro Ferreira; Rodrigues, Tiago; Cabeleira, João; Amoreira, SofiaThis paper presents the research work within the international Consortium Alliance "Universitas Montium" (UNITA) according one of many UNITA’s Research Projects: the Portuguese Project CH-RESILIENCE “Cultural Heritage: Resilience of Built Heritage and Built Environment” at the University of Beira Interior (UBI) within Universitas Montium Aliance which is included in UNITA's Research & lnnovation Cartography WP4, concerning Cultural Heritage. and soon to be included in the consortium RE-UNITA (UNITA 2.0). Within this research line two international PhD cotutelle were achieved, but also two other PhD thesis are being prepared within CH-RESILIENCE. The Department of Civil Engineering and Architecture (DECA), of the University of Beira Interior (UBI), has developed, a concern regarding Built Heritage, ln fact, over the last two decades there has been a cross-interdisciplinary interest, which has allowed a transdisciplinary research, based on the Architectural Heritage, Built Heritage, thus Cultural Heritage. Dissertation themes have focused on traditional aspects of Portuguese Architecture and Traditional Building Technologies, but also doctoral Thesis and Research Projects with competitively-based contests with funding (as The ORFEUS project which was one of the highlights of this line of research). In fact, Building Culture encompasses existing buildings, monuments, and other elements of Cultural Heritage, as well as the design and construction of contemporary buildings, infrastructures, public spaces, and landscapes. The actual technical requirements are not always compatible with older buildings. Regular maintenance of these buildings may include preservation, rehabilitation, restoration, reconstruction, refurbishment, adaptation, and interpretation of existing buildings and their environment. ln fact, this research lays on the Resilience of Built and Cultural Heritage. UNITA’s Research and innovation is centred in the needs of rural and mountain territories regarding, not only Cultural Heritage, but also Renewable Energy and Circular Economy. This paper presents four case study of international research based on the UNITA’s Alliance and interconnected with CH Resilience Project: “The Art of Place”, Cotutelle (UBI -Portugal / UNIZAR-SPAIN); “The involvement of local museums with their territorial and historical environment”, Cotutelle (UNIZAR-SPAIN / UBI -Portugal); Corbelled Dome Constructions: architectural elements of a vernacular Identity in Beira Baixa (Portugal); Resilience and Sustainability: Monastic Spaces of Cistercian origins in contemporary times. Besides these four cases it is highlighted, a fifth which is the first project, within the CH Resilience research group, in collaboration with other institutions with affinity to the theme and in the borderline of UNITA Alliance. This is the ongoing PhD thesis “Theory, Design and Construction of the Bulwark Defensive System in the Minho River Valley. From the Reign of King Philip III to the End of the Seven Years' War, 1621-1763”. Consequently, this paper will bring to discussion these specific case studies and its relationship with UNITA's Alliance. This is, through Cultural heritage it will be brought to debate methods, objectives, experiences, outputs, and discussions which allow the formation of wider networks for research and resources exchange.
- Q-Learning-Driven Enhancement of Slotted ALOHA in IEEE 802.15.4 WSNsPublication . Baptista, Amilton; Velez, Fernando J.Given the proliferation of connected devices and the prioritization of real-time data acquisition across various scenarios, enhancing the energy efficiency within Wireless Sensor Networks (WSNs) is of paramount importance. This work has focused on the IEEE 802.15.4 standard and addresses existing medium access control protocols such as CSMA or Slotted ALOHA and proposes refinements in the Slotted ALOHA protocol through incorporating techniques like Binary Exponential Backoff (BEB) and Q-learning. These enhancements have demonstrated to be promising in terms of average delay reduction, energy efficiency and bolstered network throughput. As it facilitates more efficient energy management it constitutes a robust alternative to conventional CSMA in WSN MAC sub-layer protocols.
- A Escrita Processual: A importância da planificaçãoPublication . Moreno, Mariana Pacheco; Sardinha, Maria da Graça Guilherme de Almeida; Machado, João José SerraNo âmbito do Estágio Pedagógico do Mestrado em Ensino de Português e de Espanhol no 3º Ciclo do Ensino Básico e no Ensino Secundário, no Agrupamento de Escolas do Fundão, sob orientação da professora Maria de Jesus Lopes e, integrado no programa de Português das turmas selecionadas para o efeito, procedeu-se à produção de um texto expositivo, onde metade da turma teve a oportunidade de exercitar a escrita tendo por base uma planificação e, a outra metade procedeu de imediato à escrita do texto solicitado, sem planificação. Os resultados demonstraram a importância da planificação com estratégias adequadas, para que os alunos possam tornar-se bons escreventes. Procedeu-se ainda a uma reflexão que versa sobre a prática pedagógica e a importância que esta adquire no Mestrado supracitado.
- Fibromyalgia-Related fatigue and the quality of life, depression, anxiety, self-esteem, life satisfaction and physical activity in patients with FibromyalgiaPublication . Alvarez, Marcos de Carvalho; Monteiro, Diogo Manuel Teixeira; Neiva, Henrique PereiraFibromyalgia (FM) is a chronic and persistent condition that causes physical and sensory changes. Its pathophysiology is still debated, but it is believed that the increase in symptoms is related to an imbalance of neurotransmitters or hyperexcitability of the central nervous system. Currently, 2% to 6% of the world's population has FM, with Portugal having 3% and Brazil having 2.5%. FM presents a variety of symptoms, including generalized muscle pain, excessive fatigue, anxiety and depression, reduced psychological components, and sleep problems. Due to its complexity, patients with FM often present a significant decrease in psychological components, leading to social isolation and avoidance of work responsibilities and physical activities. Therefore, this study aimed to analyze the relationship between FM-related fatigue and quality of life, anxiety, depression, self-esteem, and satisfaction with life and physical activity. The thesis was divided into three scientific studies, where the first investigated the potential role of physical activity as a mediator in the relationship between Fatigue and quality of life, and the second analyzed the direct and indirect relationships between Fatigue, anxiety, depression, self-esteem, and satisfaction with life. life and levels of physical activity and possible differences in these associations between two different cultures among patients, and the third was a comparison of symptom perceptions between two different cultures. The results of the three scientific studies included in this doctoral thesis show that: i) Although physical activity plays an extremely important role, it does not act as a mediator of the relationship between FM-related fatigue and the quality of life of its patients; ii) All associations proved to be significantly relevant, where there was also an absence of differences in terms of associations between these proposed variables in both cultures studied; iii) There were no significant differences in the perception of Fatigue, depression, anxiety, quality of life, self-esteem, satisfaction with life and physical activity between Brazilian and Portuguese patients. Combining these three studies, FM-related fatigue is an extremely relevant symptom that directly affects the psychological component of its patients, in which physical activity can have an important role in reducing and controlling negative symptoms and in increasing positive components (quality of life, self-esteem and satisfaction with life).
- Desenvolvimento Web Full-Stack na Empresa LatituddePublication . Maio, João Pedro Teixeira; Campos, Ricardo Nuno Taborda; Gouveia, João José TelesAo longo dos últimos anos o mercado de desenvolvimento web evoluiu significativamente. Impulsionado pelo crescimento da Internet, assente numa cada vez maior utilização por grande parte da população, assistimos nos últimos anos ao aparecimento de um elevado número de empresas com presença digital, tornando o desenvolvimento web uma excelente oportunidade de trabalho no domínio da informática. Em Portugal, várias empresas surgiram ao longo dos tempos com foco nesta área. A Latitudde, uma empresa da área da Tecnologia, insere-se neste domínio, tendo desenvolvido vários projetos no âmbito da web. O presente documento reflete o estágio realizado pelo aluno João Maio durante cinco meses na função de programador web full-stack em diversos projetos da empresa. O estágio teve como finalidade enriquecer o estagiário com conhecimentos deste domínio e ao mesmo tempo facultar experiência de trabalho em projetos concretos. Neste contexto, o aluno participou no desenvolvimento de dois projetos, nomeadamente o Projeto Compass e o Projeto da empresa Infraestruturas de Portugal (IP). O primeiro visou a criação de uma plataforma web que virá a ser usada por todos os membros da empresa, para a realização de tarefas que atualmente estão a ser realizadas em plataformas externas. O segundo teve como fundação a realização da migração da framework que sustenta todos os websites da empresa IP. Ao longo deste documento procede-se a uma caracterização da empresa, elencam-se os objetivos do estágio, as formações realizadas e descrevem-se as tarefas conduzidas no âmbito dos dois projetos desenvolvidos. Paralelamente é feito um levantamento do estado da arte no que ao desenvolvimento web diz respeito. Após a conclusão deste estágio o aluno foi convidado a trabalhar na empresa, num estágio profissional, continuando desta forma a ganhar experiência no mercado de trabalho.
- Blue Bolt Agency, Lda.Publication . Rodrigues, Ana Maria Amaral; Paço, Arminda Maria Finisterra doNo atual cenário, a utilização do marketing digital na estratégia empresarial desempenha um papel essencial na construção e consolidação da marca. Cada vez mais, as empresas percebem e reconhecem a importância estratégica de se adaptarem ao ambiente digital para alcançarem, de uma forma mais eficaz, o seu público-alvo. As redes sociais são um pilar fundamental no marketing digital, desempenhando um papel crucial na construção de um relacionamento com os clientes, na promoção de produtos ou serviços e na divulgação de conteúdo relevante. Para além disso, estratégias como o marketing de conteúdo, e-mail marketing e redação de artigos com a técnica de SEO são elementos-chave para aumentar a visibilidade da marca. O presente relatório de estágio resulta da realização do estágio curricular no Departamento de Marketing da empresa Blue Bolt Agency, com a duração de três meses. As atividades realizadas incidiram nas diversas componentes do Marketing Digital aplicado aos clientes da Blue Bolt Agency, e ilustram bem a relevância que este tem vindo a assumir.
- Caracterização e valorização de compostos bioativos de plantas silvestres e micropropagadas de Lavandula stoechas subsp. luisieri e Pterospartum tridentatum na conservação pós-colheita de frutosPublication . Domingues, Joana Lopes; Gonçalves, José Carlos Dias Duarte; Duarte, Ana Paula Coelho; Sousa, Fernanda Maria Grácio Delgado Ferreira deA crescente valorização de produtos naturais na alimentação tem ganho importância na comunidade científica, cada vez mais a investigação se tem direcionado para o desenvolvimento de conceitos inovadores sustentáveis ao nível alimentar e não só. Esta tendência generalizada pela sociedade de procurar, usar e consumir o que é natural deve-se não só à partilha do conhecimento científico para a sociedade, como também à urgente preocupação em mudarmos os nossos hábitos a nível alimentar, mas também à sustentabilidade dos processos industriais. As plantas aromáticas e medicinais (PAM) têm ganho especial atenção, a importância dos seus usos tradicionais tem vindo a ser confirmada, na maioria das vezes, em estudos de caracterização das propriedades biológicas. Os extratos e óleos essenciais (OEs) de PAM têm apresentado bons resultados em termos de atividades antioxidante e antimicrobiana, sendo a sua potencial aplicação na conservação de alimentos perecíveis, como é o caso de frutas e vegetais frescos, apresentando resultados promissores na substituição de conservantes químicos sintéticos e na redução do desperdício alimentar, devido à extensão de conservação dos alimentos. A Lavandula stoechas subsp. luisieri (Rozeira) Rozeira (Lsl) e a Pterospartum tridentatum L. Willk (Pt) são dois endemismos da flora portuguesa, sendo bastante referenciados em estudos etnobotânicos. Os seus extratos e OEs têm revelado resultados importantes na caracterização das suas propriedades biológicas. O presente estudo procura desenvolver protocolos de propagação in vitro destas espécies por sistemas de biorreatores de imersão temporária (BIT) e caracterizar os compostos fenólicos e voláteis dos extratos de plantas silvestres, recolhidas nas diferentes fases fenológicas e com origem em BIT. Nestes subprodutos, pretende-se avaliar as suas propriedades biológicas, nomeadamente a atividade antioxidante, antimicrobiana e a citotoxicidade. Foram realizados ensaios de conservação pós-colheita de medronho e cereja, onde, neste último, em cerejas, foram também elaboradas e caracterizadas formulações de revestimentos edíveis com incorporação de OE de Lsl. Os resultados dos ensaios de micropropagação de Lsl e Pt revelaram boas propriedades biométricas e morfológicas, obtendo taxas de multiplicação próximas ou superiores às obtidas no sistema convencional. A caracterização de extratos aquosos de Lsl revelou a presença maioritária de ácido rosmarínico em todas as fases fenológicas da planta, sendo que no extrato de BIT os compostos maioritários foram os ácidos cafeico e rosmarínico. Nos extratos aquosos de Pt os compostos fenólicos maioritários foram a rutina e quercetina, enquanto o extrato de BIT foi caracterizado por ácido elágico e rutina como compostos maioritários. Os resultados dos constituintes voláteis dos OEs de Lsl permitiram observar uma variação no perfil volátil nas diferentes fases fenológicas, sendo a fase da floração caracterizada por possuir maior teor de acetato de trans-α-necrodilo, nomeadamente no OE das folhas. Neste trabalho foi possível obter OE com origem em plantas no tratamento de BIT, sendo que o perfil volátil do OE apresentou como composto maioritário o acetato de trans-α-necrodilo, revelando alguns constituintes com maior teor comparativamente às plantas silvestres, como o α- e β-pineno, a cânfora, o acetato de lavandulilo e o viridiflorol. Os resultados permitiram concluir que os extratos aquosos e OE de Lsl e o extrato aquoso de Pt apresentaram atividade antioxidante pelos métodos testados, conferindo-lhes propriedades de potencial de aplicação como conservante. O OE de Lsl revelou potencial citotóxico em todas as concentrações testadas, enquanto nas concentrações mais baixas testadas o extrato aquoso de Pt não revelou citotoxicidade em células de fibroblastos. A atividade antimicrobiana foi confirmada no OE de Lsl, apresentando um largo espectro de ação antibacteriano e antifúngico. Foram isolados leveduras e fungos filamentosos de medronho no ensaio de conservação, permitindo a identificação de 57% dos isolados ao nível do género e espécie, destacando os géneros mais representativos como Alternaria, Cladosporium, Penicillium, Aspergillus, Aureobasidium e Saccothecium. No ensaio de conservação de cereja as formulações de revestimentos à base de quitosano e OE de Lsl apresentaram propriedades mecânicas adequadas, assim como a sua aplicação em frutos permitiu reduzir a carga microbiana inicial, contribuindo para a sua conservação e tempo de vida útil. Em suma, os extratos e OE das plantas estudadas revelaram propriedades interessantes para possível aplicação como agentes conservantes em formulações alimentares e/ou como componentes de embalagens, no entanto, validações futuras serão necessárias de forma a complementar estes resultados para o desenvolvimento de produtos bioativos na conservação de alimentos.