FE - DCTT | Documentos por Auto-Depósito
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- Enzymatic removal of plant residues from wool: Application of experimental design techniques for optimization parametersPublication . Gouveia, Isabel C.; Fiadeiro, José; Queiroz, JoãoThis study was undertaken to find the optimum conditions of a new enzymatic process to remove plant residues from wool. Commercial enzymatic preparations of Celluclast 1.5 L and Pectinex Ultra SP-L were selected in order to hydrolyze the polysaccharides in primary plant cell walls and middle lamella, resulting into more fragile residues easier to be removed. Since it was intended to define the optimal conditions for enzyme application, a four-factor central composite design was selected to study the effects of pH, temperature, enzyme concentration and wetting agent concentration, on the two selected responses, i.e., soluble reducing sugars (RS) and alkali solubility (AS) of wool to detect plant degradation and to evaluate wool quality, respectively. Results demonstrated that enzyme concentration was the most significant effect in plant residues degradation. A total enzyme concentration loading of 20 mL of both diluted enzymatic preparations in equal parts per 1 L of incubation solution (42.970 U/L of Celluclast preparation and PG 29.3 nkat/L + PME 2.537 nkat/L of Pectinex preparation), yielded an equivalent amount of 240.127 mg of glucose per 1.0 g of plant residue, at the optimal conditions: 40.56 ◦C, pH 4.0 and 1 mL Plurafac/L. SEM analysis has indicated an identical and important degradation of the plant residues, when compared to the conventional carbonization process, and wool quality has been preserved.
- Wearable Sensors for Foetal Movement Monitoring in Low Risk PregnanciesPublication . Borges, Luís M.; Araújo, Pedro; Lebres, António S.; Rente, Andreia; Salvado, Rita; Velez, Fernando J.; Martinez-de-Oliveira, J.; Barroca, Norberto; Ferro, João MiguelIn low risk pregnancies, the continuous monitoring of the foetal health is based on traditional protocols for counting the foetal movements felt by the mother. Although the maternal perception is a relevant characteristic for the evaluation of the foetal health, this kind of monitoring is hard to accomplish and being subjective can induce into errors due to mother’s anxiety and lack of concentration. Furthermore, the majority of foetal fatalities occur during the last weeks of low risk pregnancies. Therefore, it is important to obtain a universal electronic obstetric tracing, allowing for the identification of sudden changes in the foetus health, by continuously monitoring the foetus movements. The Smart-Clothing project aim has been the development of easy-to-wear belts with a telemedicine system for this purpose. One of the tried solutions is the Flex sensor belt system, which guarantees real-time and continuous foetal monitoring while creating effective interfaces for querying sensor data and store all the medical record (which can later be accessed by health professionals). Another developed belt has piezoelectric sensors incorporated onto it. The piezoelectric sensor belt has shown a high capacity to detect foetal movements, isolating them from external interferences.
- Layer-by-Layer Deposition of Antibacterial Polyelectrolytes on Cotton FibresPublication . Gomes, Ana; Mano, João F.; Queiroz, João; Gouveia, Isabel C.The introduction of molecules with biological properties on textile materials is essential for a number of biotechnological applications. With the purpose of testing new processes applied to textiles, in this study, we present the first results on the feasibility of using the Layerby-Layer (LbL) deposition process in natural fibers such as cotton, with natural polyelectrolytes like chitosan (CH) and alginic acid sodium salt (ALG), the durability of CH/ALG multilayer on cotton were evaluated. The increase of negative charges to the substrate cotton was made with NaBr and TEMPO, to ensure the success of the process of LbL. Three characterization methods to assess electrostatic LbL deposition were performed: the contact angle between a liquid (water) and the sample surface, in order to characterize the wettability of the samples with the different layers of CH and ALG; dyeing of the CH/ALG assembled cotton fabric with cationic methylene blue that shows regular changes in terms of color depth (K/S value), which indicate that the surface were alternately deposited with CH and ALG layers and, finally, the analysis by infrared spectroscopy using Fourier Transform with Attenuated Total Reflection (ATR-FTIR), to assess the changes in the interaction between CH and ALG deposited on cotton samples.
- Three New Labdanes Isolated from Eragrostis viscosaPublication . Sebastião, N'Soki; Fernandes, Nelson; Vieira, Liliana; Mendonça, António; Gaspar, Jorge; Martins, Célia; Rueff, José; Diakanamwa, Carlos; Mendonça, DinaThree new labdanes with 8α,15-epoxy ring [methyl 8α,15-epoxylabdan-16β-oate, 8α,15-epoxylabdan-16β-ol and 8α,15-epoxy-16-norlabdan-13β-ol] and five known compounds [8α,15-epoxy-16-norlabdan-13-one, 8α,15-epoxylabdan-16β-oic acid, 3β-(3'',4''-dihydroxy)(E)-cinnamoyloxylup-20(29)-ene, 3-(2',3',4',6'-tetra-O-acetyl-β-D-glucopyranosyloxy)β-sitosterol and 16-acetoxy-8α,15-epoxylabdane] were isolated from toluene and dichloromethane extracts of aerial parts of Eragrostis viscosa. The structures of all the compounds were established based on their spectroscopic data and X-ray diffraction analysis of 8α,15-epoxylabdan-16β-ol. It was also studied the genotoxicity of E. viscosa, particularly compounds 16-acetoxy-8α, 15-epoxylabdane, 8α,15-epoxy-16-norlabdan-13-one and 8α, 15-epoxilabdan-16β-ol, using a cytokinesis-block micronucleus assay and the Ames test to assess mutagenicity. Both assays were negative. Cytotoxicity was also analyzed using an MTT assay, and 8α,15-epoxy-16β-ol was shown to be the most cytotoxic of the compounds tested. E. viscosa extracts were also tested to determine their antioxidant capacities, peroxide values and total phenolic contents.
- Layer-by-layer deposition of antimicrobial polymers on cellulosic fibers: a new strategy to develop bioactive textilesPublication . Gomes, Ana; Mano, João F.; Queiroz, João; Gouveia, Isabel C.In recent years, there has been an increase of infectious diseases caused by different microorganisms and the development of antibiotic resistance. In this way, the search for new and efficient antibacterial materials is imperative. The main polysaccharides currently used in the biomedical and pharmaceutical domains are chitin and its derivative chitosan (CH) and alginates (ALG). In this study, a simple technique of Layer by Layer (LbL) of applying polycation CH and polyanion ALG was used to prepare CH/ALG multilayers on cotton samples via the electrostatic assembly with success. The CH/ALG cotton samples (functionalized) were investigated for their antibacterial properties towards Staphylococcus aureus and Klebsiella pneumonia using the international standard method JIS L 1902:2002. The antibacterial activity of the functionalized samples was tested in terms of bacteriostatic and bactericidal activity, and results showed that the samples exhibited a bacteriostatic effect on the two bacteria tested, as expected. In addition, samples with five layers (CH/ALG/CH/ALG/CH) were more effective in inhibiting bacterial growth. This new coating for cellulosic fibers is a new strategy and may open new avenues for the development of antimicrobial polymers with potential application in health-care field.
- Biofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniaePublication . Caldeira, Estela; Piskin, Erhan; Granadeiro, Luiza Breitenfeld ; Silva, Filomena; Gouveia, Isabel C.The main purpose of this work is to obtain a cotton-based textile material functionalized with l-cysteine (l-cys) to achieve an antimicrobial effect with potential application in biomedical, geriatric or pediatric textiles. The binding capacity of l-cys to cotton fibres was assessed through different functionalization strategies—surface activation and exhaustion processes. A subsequent analysis of the possible antibacterial action against Staphylococcus aureus and Klebsiella pneumoniae was performed according with the Japanese International standard (JISL, 2008). To determine the mechanism of action of l-cys on the selected strains, flow cytometry was used. The results revealed that the exhaustion process was performed with success to confer bioactivity to the treated fabric, as assessed by an effective antibacterial effect against both Gram-positive and Gram-negative bacteria, and successfully linkage of l-cys was observed via FTIR with a durable effect demonstrated after the washing tests (fastness to washing). It was also observed that l-cys exerts a bacteriostatic effect against both bacterial strains, since there were alterations in the metabolic activity ofthe microorganisms after the application of the bioactive textile which was shown by the CTC (cyanoditolyl tetrazolium chloride) staining used in flow cytometry. This study shows a new and successful biotechnological process to develop antibacterial textiles through the functionalization of cotton fibres with l-cys which presents a broad range of applications in healthcare, since l-cys is a natural antibacterial compound, non-toxic and affects pathogenic bacteria related to hospital infections.
- Spectrum opportunities for electromagnetic energy harvesting from 350 MHz to 3 GHzPublication . Tavares, Jorge Miguel da Silva; Barroca, Norberto; Saraiva, Henrique M.; Borges, Luís M.; Velez, Fernando J.; Loss, Caroline; Salvado, Rita; Pinho, Pedro; Gonçalves, Ricardo; Carvalho, Nuno BorgesThis paper presents spectrum opportunities for radio frequency (RF) energy harvesting identified through power density measurements from 350 MHz to 3 GHz. The field trials have been performed in two different cities (Covilha and Lisbon), by using the NARDA-SMR spectrum analyser with measuring antenna, and the Signal Hound spectrum analysers, respectively. The scope of our research considers RF energy harvesting devices, enabling to convert RF energy to direct current (DC), providing an alternative source to power supply wireless sensor network (WSN) devices. Printed antennas, able to operate at GSM (900/1800) bands, are proposed with gains of the order of 1.8-2.06 dBi and efficiency 77.6-84%. Guidelines for the choice of textile materials for a wearable antenna are also provided.
- New neo-Clerodanes fromTinnea antiscorbutica WelvPublication . Borges, Cristina M. P.; Mendonça, Dina; Pinheiro, Sandra C. S.; Vieira, Liliana; Mendonça, António; Gaspar, Jorge F.; Martins, Célia; Diakanamwa, Carlos; Rueff, JoséThree new neo-clerodanes, antiscorbuticane A, antiscorbuticane B and antiscorbuticane C, and known compounds glutinol, friedelin, 5,7-dihydroxyflavanone (pinocembrin), 5-hydroxy-3,6,7,4'-tetramethoxyflavone, 5-hydroxy-3,6,7,3',4'-pentamethoxyflavone (artemetin), 5,4'-dihydroxy-3,6,7,3'-tetramethoxyflavone (penduletin) and 5,3',4'-trihydroxy-3,6,7-trimethoxyflavone (chrysosplenol D), were isolated from the methanol extract of Tinnea antiscorbutica. Antiscorbuticane B exhibited no mutagenic activity at doses of up to 250 µg per plate (Ames test) and did not induce micronucleus formation in the V79 cell line at doses of up to 100 µg /mL.
- Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applicationsPublication . Gomes, Ana; Mano, João F.; Queiroz, João; Gouveia, Isabel C.A large group of low molecular weight natural compounds that exhibit antimicrobial activity has been isolated from animals and plants during the past two decades. Among them, peptides are the most widespread resulting in a new generation of antimicrobial agents with higher specific activity. In the present study we have developed a new strategy to obtain antimicrobial wound-dressings based on the incorporation of antimicrobial peptides into polyelectrolyte multilayer films built by the alternate deposition of polycation (chitosan) and polyanion (alginic acid sodium salt) over cotton gauzes. Energy dispersive X ray microanalysis technique was used to determine if antimicrobial peptides penetrated within the films. FTIR analysis was performed to assess the chemical linkages, and antimicrobial assays were performed with two strains: Staphylococcus aureus (Gram-positive bacterium) and Klebsiella pneumonia (Gram-negative bacterium). Results showed that all antimicrobial peptides used in this work have provided a higher antimicrobial effect (in the range of 4 log-6 log reduction) for both microorganisms, in comparison with the controls, and are non-cytotoxic to normal human dermal fibroblasts at the concentrations tested.
- Antimicrobial functionalization of wool: assessment of the effect of Cecropin-B and [Ala5]-Tritrp7 antimicrobial peptidesPublication . Mouro, Cláudia; Gouveia, Isabel C.This investigation provides a new strategy to impart antimicrobial properties into wool-based materials using Cecropin-B and [Ala5]-Tritrp7 antimicrobial peptides (AMPs). The process was conducted using exhaustion method at 40 °C for 1–3 h. The presence of the AMPs in the modified-wool samples was confirmed by colorimetric assay of Bradford reagent and possible changes in the morphology of the fibers and damage to its surface were analyzed by scanning electron microscopy. Results showed that 1 h were long enough for the functionalization to occur effectively and that the morphology of the fibers was not influenced by the functionalization process. Furthermore, the antimicrobial activity of the AMPs applied on wool was assessment by JIS L 1902-2002 against Staphylococcus aureus (ATCC 6538) and Klebsiella pneumoniae (ATCC 4352). The results showed that both AMPs have a high reduction in bacterial growth (Cecropin-B resulting in 71.67% reduction against S. aureus and 85.95% against K. pneumoniae. While [Ala5]-Tritrp7 resulting in 66.74% reduction against S. aureus and 88.65% against K. pneumoniae).
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