ICI - CMA | Documentos por Auto-Depósito
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- Effects of the endocrine disruptor vinclozolin in male reproduction: a systematic review and meta-analysisPublication . Feijó, Mariana; Martins, Roberta VL; Socorro, Sílvia; Pereira, L.; Correia, SaraEndocrine-disrupting chemicals have become an issue of scientific and public discussion. Vinclozolin (VNZ) is a fungicide that competitively antagonizes the binding of natural androgens to their receptor, disturbing the function of tissues that are sensitive to these hormones, as is the case of the male reproductive organs. A systematic review with meta-analyses of rodent studies was conducted to answer the following question: Does exposure to VNZ affect sperm parameters and testicular/epididymal weight? The methodology was prespecified according to the Cochrane Handbook for Systematic Reviews and PRISMA recommendations. Sixteen articles met the inclusion criteria, comprising a total of 1189 animals. The risk of publication bias was assessed using the Trim and Fill adjustment, funnel plot, and Egger regression test. Heterogeneity and inconsistency across the findings were tested using the Q-statistic and I2 of Higgins, respectively. Sensitivitywas also analyzed. Statistical analysiswas performed on Comprehensive Meta-Analysis software (Version 2.0), using random models and weighted mean differences along with a 95% confidence interval. Sperm motility, counts, daily sperm production (evidence of publication bias), and epididymis weight were decreased in VNZ-treated animals. Exposure length and dose, as well as the time point of exposure, influenced the obtained results. Despite the moderate/high heterogeneity observed, the sensitivity analysis overall demonstrated the robustness of the findings. The quality scores of the included studies were superior to 4 in a total of 9, then classified as good. The obtained data corroborate the capability of VNZ exposure to disrupt spermatogenic output and compromise male fertility.
- Action of Curcumin on Glioblastoma Growth: A Systematic Review with Meta-Analysis of Animal Model StudiesPublication . Luís, Ângelo; Amaral, Leonor; Domingues, F.C.; Pereira, L.; Cascalheira, JoséGliomas are aggressive brain tumors with poor prognosis even after surgical removal and radio-chemotherapy, stressing the urgency to find alternative therapies. Several preclinical studies evaluating the anticancer effect of curcumin in animal models of glioma are reported, but a systematic review with meta-analysis of these studies, considering the different experimental conditions used, has not been made up to this date. A search in different databases (Pubmed, Web of Science, Scopus, and SciELO) following the PRISMA statement was conducted during November 2023 to systematically identify articles assessing the effect of curcumin in murine xenograft models of glioma and identified 15 articles, which were subdivided into 24 studies. Tumor volume before and after treatment with curcumin or vehicle was extracted and the efficacy of curcumin was evaluated by performing a random effects meta-analysis of the data. Publication bias and the impact of different experimental conditions on curcumin efficacy were assessed. Treatment with curcumin decreased tumor volume. Comparing curcumin with control groups, the overall weighted standardized difference in means was −2.079 (95% CI: −2.816 to −1.341; p-value < 0.001). The curcumin effect was observed for different animal models, types of glioma cells, administration routes, and curcumin formulations. Publication bias was identified but does not invalidate curcumin’s effectiveness. The findings suggest the potential therapeutic efficacy of curcumin against glioma.
- Developing competencies through flow, gamification and cultural integration: an analysis of the potential of games in teaching/learningPublication . Lopes, Lígia; Schreurs, Sonja; Licour, Caroline; Soares, SandraMuch has been done to recover from the effects of the COVID-19 pandemic on school and university education, both in filling gaps in learning and in social integration and motivation of students post-pandemic. The need to transition from conventional teaching to remote learning has led students and teachers in a spiral of evolution in the development of digital skills due to the urgent adaptability required by the situation. The search for new technologies and didactics that seemed difficult at the time because many were not yet expecting such a sudden change, now takes advantage of that experience to create projects and courses that allow for accelerated recovery and thus transform what was once a complicated task into a new perspective on teaching and learning. One of these ways is the use of epistemic games and gamification to maintain ‘flow’. Flow is a combination of skill development through challenges and the pleasure of completing tasks. In this work, we will discuss a project that, with the union of remote and face-to-face teaching & learning, allowed university students to use the knowledge received during an intensive course on environmental radiation to design an escape room-style game with the aim of teaching students at other levels of education. CERAMUG, a Blended Intensive Programme (BIP) in partnership with ERASMUS+, aimed at two-way learning, to learn to use specific instruments for measuring natural environmental radiation and to teach, through a game, the existence of this natural radiation in order to inform and raise awareness of high school students on the subject. The aim of this course was to promote the development of competencies that allow students to succeed in their future professional challenges. The impact of the project is not only the innovative experience for students to learn specialized nuclear measurement techniques, but also to encourage other employability competencies and digital skills necessary for their careers, as well as promoting integration among students from different cultures and countries.
- Exposure to radon in buildings in the municipality of Lubango, Angola, during winter monthsPublication . Bahu, Yoenls; Kessongo, Joaquim; Peralta, Luis; Soares, SandraThe evaluation of indoor radon exposure in dwellings is done for the first time in Lubango. The corresponding annual effective dose to which the population is exposed during the wintertime was calculated. A total of 59 single-family houses and 16 public buildings were selected. The results obtained show that in 100% of the houses, the radon concentrations are below 300 Bq m−3. The values of the indoor annual effective dose vary from (2.0 ± 0.1) to (7.0 ± 0.2) mSv y−1 below the recommended maximum reference level of 10 mSv y−1.
- Therapeutic Potential of Resveratrol for Glioma: A Systematic Review and Meta-Analysis of Animal Model StudiesPublication . Luís, Ângelo; Marcelino, Helena; Domingues, F.C.; Pereira, L.; Cascalheira, JoséGliomas are aggressive malignant brain tumors, with poor prognosis despite available therapies, raising the necessity for finding new compounds with therapeutic action. Numerous preclinical investigations evaluating resveratrol’s anti-tumor impact in animal models of glioma have been reported; however, the variety of experimental circumstances and results have prevented conclusive findings about resveratrol’s effectiveness. Several databases were searched during May 2023, ten publications were identified, satisfying the inclusion criteria, that assess the effects of resveratrol in murine glioma-bearing xenografts. To determine the efficacy of resveratrol, tumor volume and animal counts were retrieved, and the data were then subjected to a random effects meta-analysis. The influence of different experimental conditions and publication bias on resveratrol efficacy were evaluated. Comparing treated to untreated groups, resveratrol administration decreased the tumor volume. Overall, the effect’s weighted standardized difference in means was −2.046 (95%CI: −3.156 to −0.936; p-value < 0.001). The efficacy of the treatment was observed for animals inoculated with both human glioblastoma or rat glioma cells and for different modes of resveratrol administration. The combined administration of resveratrol and temozolomide was more effective than temozolomide alone. Reducing publication bias did not change the effectiveness of resveratrol treatment. The findings suggest that resveratrol slows the development of tumors in animal glioma models.
- Optimization of Whey Protein-Based Films Incorporating Foeniculum vulgare Mill: Essential OilPublication . Pedro, Salomé; Pereira, L.; Domingues, F.C.; Ramos, Ana; Luís, ÂngeloPetroleum-based plastics used in food packaging are not biodegradable. They accumulate in the environment in large amounts, causing a decrease in soil fertility, jeopardizing marine habitats, and causing serious problems to human health. Whey protein has been studied for applications in food packaging, either because of its abundant availability or because it confers transparency, flexibility, and good barrier properties to packaging materials. Taking advantage of whey protein to produce new food packaging materials is a clear example of the so-called circular economy. The present work focuses on optimizing the formulation of whey protein concentrate-based films to enhance their general mechanical properties applying the Box–Behnken experimental design. Foeniculum vulgare Mill. (fennel) essential oil (EO) was incorporated into the optimized films, which were then further characterized. The incorporation of fennel EO in the films leads to a significant increase (p < 0.05) in peak elongation (from 14.03 to 31.61%) and tensile index (from 0.40 to 0.50 N.m/g). The optimized whey protein films were yellowish and very transparent (>90%). The results of the bioactive activities of the optimized films showed their ability to be applied as active materials for food packaging to improve the shelf-life of food products and also to prevent foodborne diseases associated with the growth of pathogenic microorganisms.
- The Effectiveness of Therapeutic Vaccines for the Treatment of Cervical Intraepithelial Neoplasia 3: A Systematic Review and Meta-AnalysisPublication . Ventura, Cathy; Luís, Ângelo; Soares, Christiane P.; Venuti, Aldo; Paolini, Francesca; Pereira, L.; Sousa, ÂngelaCervical cancer (CC) is a disease that affects many women worldwide, especially in low-income countries. The human papilloma virus (HPV) is the main causative agent of this disease, with the E6 and E7 oncoproteins being responsible for the development and maintenance of transformed status. In addition, HPV is also responsible for the appearance of cervical intraepithelial neoplasia (CIN), a pre-neoplastic condition burdened by very high costs for its screening and therapy. So far, only prophylactic vaccines have been approved by regulatory agencies as a means of CC prevention. However, these vaccines cannot treat HPV-positive women. A search was conducted in several databases (PubMed, Scopus, Web of Science, and ClinicalTrials.gov) to systematically identify clinical trials involving therapeutic vaccines against CIN 3. Histopathological regression data, immunological parameters, safety, DNA clearance, and vaccine efficacy were considered from each selected study, and from the 102 articles found, 8 were selected based on the defined inclusion criteria. Histopathological regression from CIN 3 to CIN < 1 was 22.1% (95% CI: 0.627–0.967; p-value = 0.024), showing a vaccine efficacy of 23.6% (95% CI; 0.666–0.876; p-value < 0.001). DNA clearance was assessed, and the risk of persistent HPV DNA was 23.2% (95% CI: 0.667–0.885; p-value < 0.001). Regarding immunological parameters, immune responses by specific T-HPV cells were more likely in vaccinated women (95% CI: 1.245–9.162; p-value = 0.017). In short, these studies favored the vaccine group over the placebo group. This work indicated that therapeutic vaccines are efficient in the treatment of CIN 3, even after accounting for publication bias.
- The Prevalence of Arcobacteraceae in Aquatic Environments: A Systematic Review and Meta-AnalysisPublication . Venâncio, Igor Miguel Antunes; Luís, Ângelo; Domingues, F.C.; Oleastro, Mónica; Pereira, L.; Ferreira, SusanaMembers of the family Arcobacteraceae are distributed widely in aquatic environments, and some of its species have been associated with human and animal illness. However, information about the diversity and distribution of Arcobacteraceae in different water bodies is still limited. In order to better characterize the health risk posed by members in the family Arcobacteraceae, a systematic review and meta-analysis-based method was used to investigate the prevalence of Arcobacteraceae species in aquatic environments based on available data published worldwide. The database search was performed using related keywords and considering studies up to February 2021. The pooled prevalence in aquatic environments was 69.2%, ranging from 0.6 to 99.9%. These bacteria have a wide geographical distribution, being found in diverse aquatic environments with the highest prevalence found in raw sewage and wastewater treatment plants (WWTP), followed by seawater, surface water, ground water, processing water from food processing plants and water for human consumption. Assessing the effectiveness of treatments in WWTP in eliminating this contamination, it was found that the wastewater treatment may not be efficient in the removal of Arcobacteraceae. Among the analyzed Arcobacteraceae species, Al. butzleri was the most frequently found species. These results highlight the high prevalence and distribution of Arcobacteraceae in different aquatic environments, suggesting a risk to human health. Further, it exposes the importance of identifying and managing the sources of contamination and taking preventive actions to reduce the burden of members of the Arcobacteraceae family.
- Desterritorialização didática de erros relacionados com (x+y)^2Publication . Peel, Misleine; Beites, P. D.; Simões, F.Será que (x+y)2=x2+y2 representa um erro? A resposta, sim ou não, depende do universo matemático em que se esteja a trabalhar. Com efeito, é um erro se, por exemplo, x e y forem números complexos arbitrários e “+” denotar a adição usual de números complexos. [...]
- Erros em esquemas de demonstração com números complexosPublication . Beites, P. D.; Branco, Maria Luísa; Costa, CecíliaAs produções escritas de estudantes podem ser objeto de análise com vista à investigação e ao ensino. Neste trabalho, um dos poucos sobre erros e números complexos, analisam- se as produções, elaboradas por estudantes do ensino superior, para uma tarefa de demonstração de um caso particular da identidade do paralelogramo. Num trabalho que pode dar pistas para a prática letiva de professores do ensino secundário e do ensino superior, procura-se classificar os esquemas de demonstração nessas produções, identificar os erros neles cometidos e relacionar estas duas vertentes de análise. A natureza da investigação é qualitativa, recorrendo à análise de conteúdo empregando, por um lado, critérios prévios de categorizações de esquemas de demonstração e, por outro, critérios novos de categorizações de erros. Constatou-se que os estudantes utilizaram, principalmente, esquemas de demonstração de convicção externa, incluídos numa subcategoria nova designada por não válidos no universo, e esquemas de demonstração dedutivos, incluídos num nível novo que se caracteriza por discernimento incipiente da compreensão da tarefa, do contexto matemático, de hipóteses e tese, e dos conhecimentos prévios a mobilizar. Os erros dominantes nas produções estão relacionados à compreensão dos conceitos de linearidade de uma função e de módulo de um número complexo, apresentando-se possíveis explicações para os mesmos. Apesar de não se identificar uma relação propriamente significativa entre os esquemas de demonstração produzidos pelos estudantes e os erros cometidos, obteve-se material para construção e discussão de questões conceituais no âmbito do tópico números complexos.